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Carbohydr Polym. 2019 Mar 1;207:684-693. doi: 10.1016/j.carbpol.2018.10.085. Epub 2018 Oct 30.

Rational design of a scalable bioprocess platform for bacterial cellulose production.

Author information

1
School of Chemical and Biomedical Engineering, Nanyang Technological University, Singapore.
2
School of Chemical and Biomedical Engineering, Nanyang Technological University, Singapore. Electronic address: slim@ntu.edu.sg.

Abstract

Bacterial cellulose (BC) has been gaining importance over the past decades as a versatile material that finds applications in diverse industries. However, a secured supply is hindered by the slow production rate and batch-to-batch variability of the yield. Here, we report a rational approach for characterising the BC production process using Design of Experiment (DoE) methodology to study the impact of different parameters on desired process attributes. Notably, we found that the carbon source used for bacterial growth significantly impacts the interplay between the process variables and affects the desired outcomes. We therefore, propose that the highest priority process outcome in this study, the yield, is a function of the carbon source and optimal reactor design. Our systematic approach has achieved projected BC yields as high as ∼40 g/L for Gluconacetobacter hansenii 53582 grown on sucrose as the carbon source compared to the widely reported yields of ∼10 g/L.

KEYWORDS:

Bacterial cellulose; Cellulose production; Design of experiments; Statistical study

PMID:
30600054
DOI:
10.1016/j.carbpol.2018.10.085
[Indexed for MEDLINE]

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