Chicken leg bone as a source of chondroitin sulfate

Carbohydr Polym. 2019 Mar 1:207:191-199. doi: 10.1016/j.carbpol.2018.11.086. Epub 2018 Nov 28.

Abstract

In this work, chondroitin sulfate (CS) was extracted from chicken leg bone soup using the heat-resin static adsorption extraction (HSAE) method. The HSAE method was optimized as follows: resin dosage, 10%; adsorption time, 4.3 h; eluent concentration, 2 M; eluent time, 1.3 h, under which the yield of CS1 from the bone soup reached 0.14% and the recovery rate was 67.35%. CS2, as reference, was obtained from the ends of chicken leg bone using enzymatic method. CS1 and CS2, together with other glycosaminoglycans, were confirmed using agarose-gel electrophoresis. The average molecular weight of CS1 and CS2 was 35.81 kDa and 37.18 kDa, respectively. The structures of CS1 and CS2 were compared using Fourier-transform infrared spectroscopy and high-performance liquid chromatography, and no significant difference was observed. Overall, the HSAE method was proposed to be a promising approach for the coproduction of CS and bone soup.

Keywords: Bone soup; Chicken leg bone; Chondroitin sulfate; Coproduction.

MeSH terms

  • Animals
  • Chickens
  • Chondroitin Sulfates / chemistry
  • Chondroitin Sulfates / isolation & purification*
  • Disaccharides / analysis
  • Electrophoresis, Agar Gel / methods
  • Leg Bones / chemistry*
  • Molecular Weight
  • Solid Phase Extraction / methods
  • Spectroscopy, Fourier Transform Infrared

Substances

  • Disaccharides
  • Chondroitin Sulfates