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Phytomedicine. 2019 Jan;52:168-177. doi: 10.1016/j.phymed.2018.09.170. Epub 2018 Sep 17.

Protective effects and active ingredients of Salvia miltiorrhiza Bunge extracts on airway responsiveness, inflammation and remodeling in mice with ovalbumin-induced allergic asthma.

Author information

1
The Second Affiliated Hospital of Nanchang University, No. 1 Minde Avenue, Donghu Dist., Nanchang 330006, People's Republic of China.
2
Hunan Provincal Maternal and Child Health Hospital, No.53 Xiangchun Road, Changsha 410008, People's Republic of China.
3
The Second Affiliated Hospital of Nanchang University, No. 1 Minde Avenue, Donghu Dist., Nanchang 330006, People's Republic of China.. Electronic address: Yanchs@outlook.com.
4
Hunan Provincal Maternal and Child Health Hospital, No.53 Xiangchun Road, Changsha 410008, People's Republic of China. Electronic address: zouwei3@mail2.sysu.edu.cn.

Abstract

BACKGROUND:

Salvia miltiorrhiza Bunge (S. miltiorrhiza), a traditional Chinese medicine, has demonstrated antioxidant, anti-inflammatory, and antibacterial activities. However, its effects against asthma that shows chronic inflammation and oxidative damage remain unknown.

PURPOSE:

To assess the effects of S. miltiorrhiza extracts on airway responsiveness, inflammation, and remodeling in ovalbumin (OVA)-induced asthmatic mice.

METHODS:

Mice with ovalbumin (OVA)-induced allergic asthma were treated with S. miltiorrhiza extracts, and airway resistance (RL) to methacholine, inflammatory cell infiltration, Th1/Th2 cytokine levels, and airway remodeling were assessed. TGF-β1-induced BEAS-2B and MRC-5 cells were used to evaluate the effects of five S. miltiorrhiza compounds on epithelial-mesenchymal transition and fibrosis.

RESULTS:

OVA-challenge resulted in remarkably increased RL, inflammatory cell infiltration, Th1/Th2 cytokine levels in BALF, goblet cell hyperplasia, collagen deposition, and airway wall thickening. Daily treatment with S. miltiorrhiza ethanolic (EE, 246 mg/kg) or water (WE, 156 mg/kg) extract significantly reduced OVA-induced airway inflammatory cell infiltration, Th1/Th2 cytokine amounts, and goblet cells hyperplasia. However, only WE remarkably decreased RL, collagen deposition, and airway wall thickening. Moreover, Chromatography showed that salvianic acid A and caffeic acid levels were much higher in WE than EE, while rosmarinic acid was slightly lower; salvianolic acid B and tanshinone IIA levels were much higher in EE than WE. Interestingly, caffeic acid and rosmarinic acid were more potent in reducing E-cadherin and vimentin levels in TGF-β1-induced BEAS-2B cells, and α-SMA and COL1A1 amounts in TGF-β1-induced MRC-5 cells.

CONCLUSIONS:

Both S. miltiorrhiza WE and EE alleviate airway inflammation in mice with OVA-sensitized allergic asthma. S. miltiorrhiza WE is more potent in reducing responsiveness and airway remodeling.

KEYWORDS:

Airway inflammation; Airway remodeling; Airway responsiveness; Allergic asthma; Ovalbumin; Salvia miltiorrhiza Bunge extracts

PMID:
30599896
DOI:
10.1016/j.phymed.2018.09.170
[Indexed for MEDLINE]

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