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Biomed Pharmacother. 2019 Mar;111:579-587. doi: 10.1016/j.biopha.2018.12.074. Epub 2018 Dec 31.

Protective effects of p-coumaric acid against oxidant and hyperlipidemia-an in vitro and in vivo evaluation.

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Center for Drug Design, College of Pharmacy, University of Minnesota, Minneapolis, MN 55455, USA; Department of Food Science and Engineering, School of Science and Engineering, Jinan University, Guangzhou 510632, Guangdong, China.
Center for Drug Design, College of Pharmacy, University of Minnesota, Minneapolis, MN 55455, USA.
Agro-Food Science and Technology Research Institute, Guangxi Academy of Agricultural Sciences, Nanning 530007, Guangxi, China; Guangxi Key Laboratory of Fruits and Vegetables Storage-processing Technology, Nanning 530007, Guangxi, China.
Center for Excellence in Post-Harvest Technologies, North Carolina A&T State University, the North Carolina Research Campus, 500 Laureate Way, Kannapolis, NC 2802, USA.
Formula-Pattern Research Center, School of Traditional Chinese Medicine, Jinan University, Guangzhou 510632, Guangdong, China.
National Engineering Laboratory for Rice and By-product Deep Processing, College of Food Science and Engineering, Center South University of Forestry and Technology, Changsha 410004, China.
College of Food Science and Engineering, Nanjing University of Finance and Economics, Nanjing 210023, China.
State Key Laboratory of Food Science and Technology, Synergetic Innovation Center of Food Safety and Nutrition, School of Food Science and Technology, Jiangnan University, Wuxi 214122, Jiangsu, China.
Department of Chemistry, Temple University, Philadelphia, 19122, USA. Electronic address:


Dietary phenols are antioxidants with diverse physiological functions that are beneficial for human health. The objective of this research work was to investigate antioxidant activity of p-coumaric acid (p-CA) using four in vitro methods, the protective effects against oxidative stress in PC12 cells, and hypolipidemic effects on High fat-diet (HFD) mice model. The p-CA exhibited moderate antioxidant activity in the selected in vitro assay. The highest chelating activity of p-CA at 50 μg/mL was found to be 52.22%. Pretreatment with p-CA significantly enhanced cell viability of PC12 cell and suppressed AAPH-induced intracellular ROS generation and AAPH-induced LDH release. The hypolipidemic effects of p-CA (100 mg/kg BW) was directly linked to the increased expression of nuclear factor erythroid 2-related factor (Nrf2) by 2.0-fold, Glutathione peroxidase (Gpx) by 3.8-fold, Superoxide dismutase (SOD-1) by 1.6-fold, Heme oxygenase (HO-1) by 1.72-fold and NAD(P)H Quinone Dehydrogenase 1 (NQO-1) by 1.5-fold compared with HFD group. In addition to these effects, p-CA decreased total cholesterol and atherosclerosis index levels, and increased catalase (CAT) level in serum, total antioxidant capacity (T-AOC) and glutathione peroxidase (GSH-Px) levels in liver as compared HFD group. Administration of p-CA also promoted the recovery of hyperlipidemia steatohepatitis in mice by ameliorating lipid peroxidation. These results suggested that p-CA is a potent antioxidant with potential therapeutic efficacy for treating hyperlipidemia symptoms.


Antioxidant activity; High fat-diet mice model; Oxidative stress; PC12 cells; p-Courmaric acid

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