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Biomed Pharmacother. 2019 Mar;111:579-587. doi: 10.1016/j.biopha.2018.12.074. Epub 2018 Dec 31.

Protective effects of p-coumaric acid against oxidant and hyperlipidemia-an in vitro and in vivo evaluation.

Author information

1
Center for Drug Design, College of Pharmacy, University of Minnesota, Minneapolis, MN 55455, USA; Department of Food Science and Engineering, School of Science and Engineering, Jinan University, Guangzhou 510632, Guangdong, China.
2
Center for Drug Design, College of Pharmacy, University of Minnesota, Minneapolis, MN 55455, USA.
3
Agro-Food Science and Technology Research Institute, Guangxi Academy of Agricultural Sciences, Nanning 530007, Guangxi, China; Guangxi Key Laboratory of Fruits and Vegetables Storage-processing Technology, Nanning 530007, Guangxi, China.
4
Center for Excellence in Post-Harvest Technologies, North Carolina A&T State University, the North Carolina Research Campus, 500 Laureate Way, Kannapolis, NC 2802, USA.
5
Formula-Pattern Research Center, School of Traditional Chinese Medicine, Jinan University, Guangzhou 510632, Guangdong, China.
6
National Engineering Laboratory for Rice and By-product Deep Processing, College of Food Science and Engineering, Center South University of Forestry and Technology, Changsha 410004, China.
7
College of Food Science and Engineering, Nanjing University of Finance and Economics, Nanjing 210023, China.
8
State Key Laboratory of Food Science and Technology, Synergetic Innovation Center of Food Safety and Nutrition, School of Food Science and Technology, Jiangnan University, Wuxi 214122, Jiangsu, China.
9
Department of Chemistry, Temple University, Philadelphia, 19122, USA. Electronic address: guanyifu200181@163.com.

Abstract

Dietary phenols are antioxidants with diverse physiological functions that are beneficial for human health. The objective of this research work was to investigate antioxidant activity of p-coumaric acid (p-CA) using four in vitro methods, the protective effects against oxidative stress in PC12 cells, and hypolipidemic effects on High fat-diet (HFD) mice model. The p-CA exhibited moderate antioxidant activity in the selected in vitro assay. The highest chelating activity of p-CA at 50 μg/mL was found to be 52.22%. Pretreatment with p-CA significantly enhanced cell viability of PC12 cell and suppressed AAPH-induced intracellular ROS generation and AAPH-induced LDH release. The hypolipidemic effects of p-CA (100 mg/kg BW) was directly linked to the increased expression of nuclear factor erythroid 2-related factor (Nrf2) by 2.0-fold, Glutathione peroxidase (Gpx) by 3.8-fold, Superoxide dismutase (SOD-1) by 1.6-fold, Heme oxygenase (HO-1) by 1.72-fold and NAD(P)H Quinone Dehydrogenase 1 (NQO-1) by 1.5-fold compared with HFD group. In addition to these effects, p-CA decreased total cholesterol and atherosclerosis index levels, and increased catalase (CAT) level in serum, total antioxidant capacity (T-AOC) and glutathione peroxidase (GSH-Px) levels in liver as compared HFD group. Administration of p-CA also promoted the recovery of hyperlipidemia steatohepatitis in mice by ameliorating lipid peroxidation. These results suggested that p-CA is a potent antioxidant with potential therapeutic efficacy for treating hyperlipidemia symptoms.

KEYWORDS:

Antioxidant activity; High fat-diet mice model; Oxidative stress; PC12 cells; p-Courmaric acid

PMID:
30599319
DOI:
10.1016/j.biopha.2018.12.074
[Indexed for MEDLINE]
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