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Methods. 2019 Apr 15;159-160:138-145. doi: 10.1016/j.ymeth.2018.12.010. Epub 2018 Dec 29.

Using time-lapse fluorescence microscopy to study gene regulation.

Author information

1
Department of Physics, The Pennsylvania State University, University Park, PA 16802, United States; Center for Eukaryotic Gene Regulation, The Pennsylvania State University, University Park, PA 16802, United States.
2
Department of Physics, The Pennsylvania State University, University Park, PA 16802, United States; Department of Biochemistry and Molecular Biology, The Pennsylvania State University, University Park, PA 16802, United States; Center for Eukaryotic Gene Regulation, The Pennsylvania State University, University Park, PA 16802, United States. Electronic address: lub15@psu.edu.

Abstract

Time-lapse fluorescence microscopy is a powerful tool to study gene regulation. By probing fluorescent signals in single cells over extended period of time, this method can be used to study the dynamics, noise, movement, memory, inheritance, and coordination, of gene expression during cell growth, development, and differentiation. In combination with a flow-cell device, it can also measure gene regulation by external stimuli. Due to the single cell nature and the spatial/temporal capacity, this method can often provide information that is hard to get using other methods. Here, we review the standard experimental procedures and new technical developments in this field.

PMID:
30599195
PMCID:
PMC6589118
[Available on 2020-04-15]
DOI:
10.1016/j.ymeth.2018.12.010

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