Bonding durability, antibacterial activity and biofilm pH of novel adhesive containing antibacterial monomer and nanoparticles of amorphous calcium phosphate

Yuncong Li; Xiaoyi Hu; Jianping Ruan; Dwayne D Arola; Chao Ji; Michael D Weir; Thomas W Oates; Xiaofeng Chang; Ke Zhang; Hockin H K Xu

doi:10.1016/j.jdent.2018.12.013

Bonding durability, antibacterial activity and biofilm pH of novel adhesive containing antibacterial monomer and nanoparticles of amorphous calcium phosphate

J Dent. 2019 Feb:81:91-101. doi: 10.1016/j.jdent.2018.12.013. Epub 2018 Dec 30.

Authors

Yuncong Li¹, Xiaoyi Hu², Jianping Ruan², Dwayne D Arola³, Chao Ji⁴, Michael D Weir⁴, Thomas W Oates⁴, Xiaofeng Chang⁵, Ke Zhang⁶, Hockin H K Xu⁷

Affiliations

¹ Clinical Research Center of Shaanxi Province for Dental and Maxillofacial Diseases, Key Laboratory of Shaanxi Province for Craniofacial Precision Medicine Research, College of Stomatology, Xi'an Jiaotong University, Xi'an, Shaanxi, 710004, China; Department of Advanced Oral Sciences & Therapeutics, University of Maryland School of Dentistry, Baltimore, MD, 21201, USA.
² Clinical Research Center of Shaanxi Province for Dental and Maxillofacial Diseases, Key Laboratory of Shaanxi Province for Craniofacial Precision Medicine Research, College of Stomatology, Xi'an Jiaotong University, Xi'an, Shaanxi, 710004, China.
³ Department of MaterialsScience and Engineering, University of Washington, Seattle, WA, 98195, USA.
⁴ Department of Advanced Oral Sciences & Therapeutics, University of Maryland School of Dentistry, Baltimore, MD, 21201, USA.
⁵ Clinical Research Center of Shaanxi Province for Dental and Maxillofacial Diseases, Key Laboratory of Shaanxi Province for Craniofacial Precision Medicine Research, College of Stomatology, Xi'an Jiaotong University, Xi'an, Shaanxi, 710004, China. Electronic address: changxf@xjtu.edu.cn.
⁶ Department of Advanced Oral Sciences & Therapeutics, University of Maryland School of Dentistry, Baltimore, MD, 21201, USA; Department of Orthodontics, School of Stomatology, Capital Medical University, Beijing, China. Electronic address: tuzizhangke@163.com.
⁷ Department of Advanced Oral Sciences & Therapeutics, University of Maryland School of Dentistry, Baltimore, MD, 21201, USA; Center for Stem Cell Biology & Regenerative Medicine, University of Maryland School of Medicine, Baltimore, MD, 21201, USA; Department of Mechanical Engineering, University of Maryland Baltimore County, Baltimore County, MD, 21250, USA. Electronic address: hxu@umaryland.edu.

PMID: 30599165
DOI: 10.1016/j.jdent.2018.12.013

Abstract

Objectives: The dentin bonding often fails over time, leading to secondary caries and restoration failure. The objectives of this study were to develop an adhesive with dimethylaminohexadecyl methacrylate (DMAHDM) and nanoparticles of amorphous calcium phosphate (NACP), and investigate the effects of storage in artificial saliva for six months on the bonding durability, antibacterial activity, ion release and biofilm pH properties for the first time.

Methods: DMAHDM was added at 5% (by mass) to Scotchbond Primer and Adhesive (SBMP). NACP was added at 10%, 20%, and 30% to SBMP adhesive. Dentin bonding durability, antibacterial activity against Streptococcus mutans biofilms, and calcium (Ca) and phosphate (P) ion liberation properties were investigated after 1 day and 6months of storage in artificial saliva.

Results: Dentin bond strength (n = 50) had 25% loss after 6 months of aging for SBMP control. However, SBMP + DMAHDM+10NACP and SBMP + DMAHDM+20NACP showed no loss in bond strength after storage in artificial saliva for 6 months. The DMAHDM + NACP incorporation method dramatically reduced the bioﬁlm metabolic activity and acid production, and decreased the bioﬁlm CFU by four orders of magnitude, compared to SBMP control, even after 6 months of aging (p < 0.05). DMAHDM + NACP had long-lasting Ca and P ion releases, and raised the biofilm pH to 6.8, while the control group had a cariogenic biofilm pH of 4.5.

Conclusions: Incorporating DMAHDM + NACP in bonding agent yielded potent and long-lasting antibacterial activity and ions liberation ability, and much higher long-term dentin bond strength after 6-month of aging. The new bonding agent is promising to inhibit caries at the restoration margins and increase the resin-dentin bonding longevity.

Clinical significance: The novel bioactive adhesive is promising to protect tooth structures from biofilm acids and secondary caries. NACP and DMAHDM have great potential for applications to a wide range of dental materials to reduce plaque and achieve therapeutic effects.

Keywords: Antibacterial monomer; Artificial saliva aging; Calcium phosphate nanoparticles; Dentin bonding durability; Oral biofilms; Remineralization.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Anti-Bacterial Agents*
Biofilms
Calcium Phosphates*
Dental Cements*
Hydrogen-Ion Concentration
Materials Testing
Methacrylates
Nanoparticles*

Substances

Anti-Bacterial Agents
Calcium Phosphates
Dental Cements
Methacrylates
amorphous calcium phosphate