Format

Send to

Choose Destination
J Venom Anim Toxins Incl Trop Dis. 2018 Dec 22;24:41. doi: 10.1186/s40409-018-0181-8. eCollection 2018.

Next-generation sequencing analysis reveals high bacterial diversity in wild venomous and non-venomous snakes from India.

Author information

1
AgriGenome Labs Pvt. Ltd., SmartCity Kochi, Kakkanad, Kerala 682042 India.
2
SciGenom Research Foundation, Cheruthuruthy, Kerala 679531 India.
3
Department of Forests and Wildlife, Sulthan Batheri, Wayanad District, Kerala 673592 India.
4
4Medgenome Labs Pvt. Ltd., Narayana Health City, Bommasandra, Bengaluru, Karnataka 560099 India.

Abstract

Background:

The oral cavities of snakes are replete with various types of bacterial flora. Culture-dependent studies suggest that some of the bacterial species are responsible for secondary bacterial infection associated with snakebite. A complete profile of the ophidian oral bacterial community has been unreported until now. Therefore, in the present study, we determined the complete bacterial compositions in the oral cavity of some snakes from India.

Methods:

Total DNA was isolated from oral swabs collected from three wild snake species (Indian Cobra, King Cobra and Indian Python). Next, the DNA was subjected to PCR amplification of microbial 16S rRNA gene using V3-region-specific primers. The amplicons were used for preparation of DNA libraries that were sequenced on an Illumina MiSeq platform.

Results:

The cluster-based taxonomy analysis revealed that Proteobacteria and Actinobacteria were the most predominant phyla present in the oral cavities of snakes. This result indicates that snakes show more similarities to birds than mammals as to their oral bacterial communities. Furthermore, our study reports all the unique and common bacterial species (total: 147) found among the oral microbes of snakes studied, while the majority of commonly abundant species were pathogens or opportunistic pathogens to humans. A wide difference in ophidian oral bacterial flora suggests variation by individual, species and geographical region.

Conclusion:

The present study would provide a foundation for further research on snakes to recognize the potential drugs/antibiotics for the different infectious diseases.

KEYWORDS:

Hypervariable region; Microbial community; Next-generation sequencing; Venomous snake

Conflict of interest statement

The snakes were handled carefully throughout the exercise and after the collection of oral samples, they were released back into the wild. The experiments were conducted under the guidelines of the Department of Forests and Wildlife, Kerala state, India.Not applicable.The authors declare that they have no competing interests.Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Supplemental Content

Full text links

Icon for BioMed Central Icon for PubMed Central
Loading ...
Support Center