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Nat Struct Mol Biol. 2019 Jan;26(1):14-24. doi: 10.1038/s41594-018-0173-y. Epub 2018 Dec 31.

CRISPR RNA-guided autonomous delivery of Cas9.

Author information

1
Department of Microbiology and Immunology, Montana State University, Bozeman, Montana, USA.
2
Department of Microbiology and Immunology, Montana State University, Bozeman, Montana, USA. bwiedenheft@gmail.com.

Abstract

Cas9 is an endonuclease that can be programed to autonomously deliver diverse effectors to specified genetic addresses. High-resolution structures of this protein and its associated CRISPR RNA guide explain the molecular mechanisms of CRISPR-RNA-guided DNA recognition and provide a molecular blueprint that has facilitated structure-guided functional remodeling. Here we retrace events that led from early efforts to understand the central role of Cas9 in CRISPR-mediated adaptive immunity to contemporary efforts aimed at developing and deploying this enzyme for programmable genetic editing.

PMID:
30598555
DOI:
10.1038/s41594-018-0173-y

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