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Molecules. 2018 Dec 30;24(1). pii: E120. doi: 10.3390/molecules24010120.

Bioactivity-Guided Purification of Novel Herbal Antioxidant and Anti-NO Compounds from Euonymus laxiflorus Champ.

Nguyen VB1, Wang SL2,3, Nguyen AD4, Lin ZH5, Doan CT6,7, Tran TN8,9, Huang HT10,11, Kuo YH12,13,14.

Author information

1
Institute of Research and Development, Duy Tan University, Da Nang 550000, Vietnam. bondhtn@gmail.com.
2
Department of Chemistry, Tamkang University, New Taipei City 25137, Taiwan. sabulo@mail.tku.edu.tw.
3
Life Science Development Center, Tamkang University, New Taipei City 25137, Taiwan. sabulo@mail.tku.edu.tw.
4
Institute of Biotechnology and Environment, Tay Nguyen University, Buon Ma Thuot 630000, Vietnam. nadzungtaynguyenuni@yahoo.com.vn.
5
Division of Chinese Materia Medica Development, National Research Institute of Chinese Medicine, Taipei 11221, Taiwan. tiger77749@gmail.com.
6
Department of Chemistry, Tamkang University, New Taipei City 25137, Taiwan. doanthng@gmail.com.
7
Department of Science and Technology, Tay Nguyen University, Buon Ma Thuot 630000, Vietnam. doanthng@gmail.com.
8
Department of Chemistry, Tamkang University, New Taipei City 25137, Taiwan. tranngoctnu@gmail.com.
9
Department of Science and Technology, Tay Nguyen University, Buon Ma Thuot 630000, Vietnam. tranngoctnu@gmail.com.
10
Division of Chinese Materia Medica Development, National Research Institute of Chinese Medicine, Taipei 11221, Taiwan. kk49310953@nricm.edu.tw.
11
Department of Biochemical Science and Technology, National Taiwan University, Taipei 106, Taiwan. kk49310953@nricm.edu.tw.
12
Division of Chinese Materia Medica Development, National Research Institute of Chinese Medicine, Taipei 11221, Taiwan. kuoyh@nricm.edu.tw.
13
Graduate Institute of Integrated Medicine, College of Chinese Medicine, China Medical University, Taichung 40402, Taiwan. kuoyh@nricm.edu.tw.
14
Ph.D. Program for Clinical Drug Development of Chinese Herbal Medicine, College of Pharmacy, Taipei Medical University, Taipei 11031, Taiwan. kuoyh@nricm.edu.tw.

Abstract

Euonymus laxiflorus Champ., a medicinal herb collected in Vietnam, has been reported to show several potent bioactivities, including anti-NO, enzyme inhibition, hypoglycemic and antidiabetic effects. Recently, the antioxidant activity of Euonymus laxiflorus Champ. trunk bark (ELCTB) has also been reported. However, the active antioxidant and anti-NO constituents existing in ELCTB have not been reported in the literature. The objective of this study was to purify the active antioxidants from ELCTB and investigate the anti-NO effect of the major constituents. Twenty-two phenolics isolated from ELCTB, including 12 compounds newly isolated in this study (112) and 10 constituents obtained from our previous work, were evaluated for their antioxidant activity. Of these, 12 compounds (46, 9, 1315, 1822) showed a potent antioxidant capacity (FRS50 = 7.8⁻58.11 µg/mL), in comparison to α-tocopherol (FRS50 = 23 µg/mL). In the anti-NO activity tests, Walterolactone A (1a) and B (1b) β-d-glucopyranoside (13) demonstrated the most effective and comparable activity to that of quercetin with max inhibition and IC50 values of 100%, 1.3 µg/mL, and 100%, 1.21 µg/mL, respectively. The crude extract and its major compounds showed no cytotoxicity on normal cells. Notably, three constituents (9, 11, and 12) were identified as new compounds, another three constituents, including 1, 7, and 8, were found to be new natural products, constituents 9 and 13 were determined to be new antioxidants, and compound 13 was reported to have novel potent anti-NO activity for the first time. The results of this study contribute to the enrichment of new natural products and compounds, as well as the novel biological activity of constituents isolated from Euonymus laxiflorus Champ. The current study also indicates ELCTB as a rich natural source of active phenolics. It is suggested that ELCTB could be developed as a health food with promising antioxidant and anti-NO effects, as well as other beneficial biological activities.

KEYWORDS:

Euonymus laxiflorus; Walterolactone A (1a) and B (1b) β-d-glucopyranoside; anti-NO; antioxidants; bioactive compounds

PMID:
30598014
PMCID:
PMC6337563
DOI:
10.3390/molecules24010120
[Indexed for MEDLINE]
Free PMC Article

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