Identification and characterization of a non-interferon antileishmanial macrophage activating factor (antileishmanial MAF)

A Van Niel; S E Zacks; J R David; H G Remold; W Y Weiser

doi:10.1007/978-1-4757-5421-6_23

Identification and characterization of a non-interferon antileishmanial macrophage activating factor (antileishmanial MAF)

Adv Exp Med Biol. 1988:239:231-7. doi: 10.1007/978-1-4757-5421-6_23.

Authors

A Van Niel¹, S E Zacks, J R David, H G Remold, W Y Weiser

Affiliation

¹ Department of Medicine, Harvard Medical School, Boston, Massachusetts.

PMID: 3059767
DOI: 10.1007/978-1-4757-5421-6_23

Abstract

A non-interferon lymphokine elaborated from PHA and Con A-stimulated human T-cell hybridoma, T-CEMA, has been found to activate monocyte-derived macrophages for the intracellular killing of L. donovani (antileishmanial MAF). This T-cell hybridoma derived antileishmanial MAF which has an apparent mw of 65,000 and pI of 5.3-5.6, contains neither antiviral activity nor colony stimulating activity. Furthermore, antileishmanial MAF is not neutralized by anti-MIF, anti-IFN-gamma or anti-GM-CSF antibodies.

MeSH terms

Animals
Antibodies, Monoclonal
Cell Line
Humans
Hybridomas / analysis
Isoelectric Point
Leishmania donovani / drug effects
Leishmania donovani / immunology*
Lymphokines / isolation & purification
Lymphokines / physiology*
Macrophage Activation* / drug effects
Macrophage Migration-Inhibitory Factors / immunology
Macrophage Migration-Inhibitory Factors / isolation & purification
T-Lymphocytes / analysis

Substances

Antibodies, Monoclonal
Lymphokines
Macrophage Migration-Inhibitory Factors