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Methods Mol Biol. 2019;1911:247-261. doi: 10.1007/978-1-4939-8976-8_17.

Investigating Hepatitis C Virus Infection Using Super-Resolution Microscopy.

Author information

1
Quantitative Imaging and Nanobiophysics Group, MRC Laboratory for Molecular Cell Biology, Department of Cell and Developmental Biology, University College London, London, UK.
2
Division of Infection and Immunity, Institute of Immunity and Transplantation, University College London, London, UK. j.grove@ucl.ac.uk.

Abstract

Super-resolution microscopy (SRM) can provide a window on the nanoscale events of virus replication. Here we describe a protocol for imaging hepatitis C virus-infected cells using localization SRM. We provide details on sample preparation, immunostaining, data collection, and super-resolution image reconstruction. We have made all efforts to generalize the protocol to make it accessible to all budding super-resolution microscopists.

KEYWORDS:

Hepatitis; Imaging; Microscopy; Super-resolution; Virus

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