Format

Send to

Choose Destination
Methods Mol Biol. 2019;1911:209-217. doi: 10.1007/978-1-4939-8976-8_14.

Measuring Hepatitis C Virus Envelopment by Using a Proteinase K Protection Assay.

Author information

1
Department of Molecular Genetics and Microbiology, Duke University Medical Center, Durham, NC, USA.
2
Department of Molecular Genetics and Microbiology, Duke University Medical Center, Durham, NC, USA. stacy.horner@duke.edu.
3
Department of Medicine, Duke University Medical Center, Durham, NC, USA. stacy.horner@duke.edu.

Abstract

The infectious virion of hepatitis C virus (HCV) is made up of the viral nucleocapsid surrounded by an envelope that contains an ER-derived membrane bilayer, cellular lipids, and the viral E1 and E2 glycoproteins. Because the infectious HCV particle contains both protein and lipid layers, selective disruption of these layers and analysis for the presence or absence of resulting virion components can be used to study the virion assembly process. This chapter describes an experimental method to measure HCV virion envelopment, which can reveal the mechanisms of how specific viral protein-protein interactions and host factors contribute to the process of HCV envelopment.

KEYWORDS:

Electroporation; Envelopment; Hepatitis C virus; Immunoblot; Protection assay; Proteinase K

PMID:
30593628
PMCID:
PMC6357228
[Available on 2020-01-01]
DOI:
10.1007/978-1-4939-8976-8_14

Supplemental Content

Full text links

Icon for Springer
Loading ...
Support Center