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Anticancer Res. 2019 Jan;39(1):173-175. doi: 10.21873/anticanres.13094.

Twist1 Correlates With Epithelial-Mesenchymal Transition Markers Fibronectin and Vimentin in Adrenocortical Tumors.

Author information

1
Endocrine Oncology Unit, Brazilian National Cancer Institute - INCA, Rio de Janeiro, Brazil danielbulzico@gmail.com.
2
Endocrinology Section, Federal Hospital of Lagoa, Rio de Janeiro, Brazil.
3
Stem Cell Laboratory, Center for Bone Marrow Transplants, Brazilian National Cancer Institute - INCA, Rio de Janeiro, Brazil.
4
Division of Pathology, Brazilian National Cancer Institute - INCA, Rio de Janeiro, Brazil.
5
Department of Internal Medicine and Endocrinology Section, Medical School and Clementino Fraga Filho University Hospital, Rio de Janeiro Federal University, Rio de Janeiro, Brazil.

Abstract

BACKGROUND/AIM:

Although the knowledge regarding adrenocortical carcinomas (ACC) tumorigenesis has significantly improved during the last decade, it still remains to be completely determined. Epithelial-mesenchymal transition (EMT) is a well described transcription factor induced process, postulated as an essential step toward cancer progression and metastasis development. In this context, Twist1 has been described as the EMT master-regulator. The aim of this study was to assess the association among Twist1, fibronectin, vimentin and E-cadherin gene expression in adrenocortical tumor samples.

MATERIALS AND METHODS:

Twist1, fibronectin, vimentin and E-cadherin gene expression in 18 adrenal adenomas, 18 ACC, and 24 childhood onset adrenocortical tumors were assessed in formalin-fixed paraffin-embedded tissues. The fold expression was calculated according to the 2ΔCt method.

RESULTS:

A significant correlation between mRNA levels of Twist1, fibronectin and vimentin was evident. Although their expression was inversely proportional, no association was observed between Twist1 and E-cadherin expression.

CONCLUSION:

The expression of Twist1, the major regulator of EMT, is directly correlated to the expression of mesenchymal markers fibronectin and vimentin in ACC samples.

KEYWORDS:

Adrenocortical carcinoma; epithelial-mesenchymal transition; transcriptional factor

PMID:
30591455
DOI:
10.21873/anticanres.13094
[Indexed for MEDLINE]

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