Format

Send to

Choose Destination
J Med Microbiol. 1988 Dec;27(4):227-32.

Characterisation and cellular localisation of the immunodominant 47-Kda antigen of Candida albicans.

Author information

1
Department of Medical Microbiology, St Bartholomew's Hospital, West Smithfield, London.

Abstract

The 47-Kda component of Candida albicans is an immunodominant antigen in the serology of systemic candidosis. Immuno-electronmicroscopy with an affinity-purified antibody to the 47-Kda antigen showed that it was present in the cytoplasm and cell wall of both yeast and mycelial cells. It was found in discrete areas on the inner and outer borders of the cell wall and was mainly located within the wall rather than exposed on the outer surface. Sometimes it appeared to be in channels across the cell wall. In the cytoplasm, it was usually near the cytoplasmic membrane and occasionally appeared in vesicular areas. It was not detected in the nucleus or mitochondria. The 47-Kda antigen did not bind to Concanavalin A, and antigenicity was lost after protease digestion. Peptide mapping suggested that the antigen was highly conserved between different strains of C. albicans.

PMID:
3058979
DOI:
10.1099/00222615-27-4-227
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Ingenta plc
Loading ...
Support Center