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Mar Drugs. 2018 Dec 24;17(1). pii: E10. doi: 10.3390/md17010010.

UPLC⁻MS/MS Identification of Sterol Sulfates in Marine Diatoms.

Author information

1
Bio-Organic Chemistry Unit, Institute of Biomolecular Chemistry CNR, Via Campi Flegrei 34, Pozzuoli, 80078 Naples, Italy. nuzzo.genoveffa@icb.cnr.it.
2
Bio-Organic Chemistry Unit, Institute of Biomolecular Chemistry CNR, Via Campi Flegrei 34, Pozzuoli, 80078 Naples, Italy. carmen.gallo@icb.cnr.it.
3
Bio-Organic Chemistry Unit, Institute of Biomolecular Chemistry CNR, Via Campi Flegrei 34, Pozzuoli, 80078 Naples, Italy. gdippolito@icb.cnr.it.
4
Bio-Organic Chemistry Unit, Institute of Biomolecular Chemistry CNR, Via Campi Flegrei 34, Pozzuoli, 80078 Naples, Italy. emanzo@icb.cnr.it.
5
Bio-Organic Chemistry Unit, Institute of Biomolecular Chemistry CNR, Via Campi Flegrei 34, Pozzuoli, 80078 Naples, Italy. nadia.ruocco@gmail.com.
6
Department of Marine Biotechnology, Stazione Zoologica Anton Dohrn, 80121 Naples, Italy. nadia.ruocco@gmail.com.
7
Department of Biology and Evolution of Marine Organisms, Stazione Zoologica Anton Dohrn, Villa Comunale, 80121 Napoli, Italy. ennio.erre@gmail.com.
8
Department of Biology and Evolution of Marine Organisms, Stazione Zoologica Anton Dohrn, Villa Comunale, 80121 Napoli, Italy. ylenia@szn.it.
9
Department of Marine Biotechnology, Stazione Zoologica Anton Dohrn, 80121 Naples, Italy. mcosta@szn.it.
10
Department of Marine Biotechnology, Stazione Zoologica Anton Dohrn, 80121 Naples, Italy. vzupo@szn.it.
11
Bio-Organic Chemistry Unit, Institute of Biomolecular Chemistry CNR, Via Campi Flegrei 34, Pozzuoli, 80078 Naples, Italy. angela.sardo@icb.cnr.it.
12
Bio-Organic Chemistry Unit, Institute of Biomolecular Chemistry CNR, Via Campi Flegrei 34, Pozzuoli, 80078 Naples, Italy. afontana@icb.cnr.it.

Abstract

Diatoms are unicellular eukaryotic organisms that play a key ecological and biogeochemical role in oceans as major primary producers. Recently, these microalgae have also attracted interest as a promising source of functional products with widespread relevance. Progress in the knowledge of cell and molecular biology of diatoms is envisaged as a key step to understanding regulation of their life cycle in marine environments as well as facilitating their full and profitable exploitation by biotechnological platforms. Recently, we identified sterol sulfates (StS) as regulatory molecules of cell death in the diatom Skeletonema marinoi. As these compounds may have a general role in diatom physiology and chemical signals in aquatic systems, we investigated a suitable tool for their analysis in laboratory and field samples. Herein, we describe a sensitive, fast, and efficient ultra performance liquid chromatography⁻mass spectrometry (UPLC⁻MS) method for qualitative and quantitative analysis of StS from crude extract of diatoms and other microalgae. The method was applied to 13 different strains of our collection of marine protists. This first study suggested a species-specific distribution of StS and identified the sulfated derivatives of 24-methylene cholesterol and 24-methyl cholesterol as the most common members in diatoms.

KEYWORDS:

chromatography; diatom; dinoflagellate; marine organism; mass spectrometry; natural product; secondary metabolite; sterol; sulfotransferase; terpene

PMID:
30586934
PMCID:
PMC6356472
DOI:
10.3390/md17010010
[Indexed for MEDLINE]
Free PMC Article

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