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Crit Rev Food Sci Nutr. 2018 Dec 22:1-12. doi: 10.1080/10408398.2018.1518896. [Epub ahead of print]

Measurements of lycopene contents in fruit: A review of recent developments in conventional and novel techniques.

Hussain A1,2,3, Pu H1,2,3, Sun DW1,2,3,4.

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a School of Food Science and Engineering , South China University of Technology , Guangzhou 510641 , PR China.
b Academy of Contemporary Food Engineering , South China University of Technology, Guangzhou Higher Education Mega Center , Guangzhou 510006 , PR China.
c Engineering and Technological Research Centre of Guangdong Province on Intelligent Sensing and Process Control of Cold Chain Foods , Guangzhou Higher Education Mega Centre , Guangzhou , China.
d Food Refrigeration and Computerized Food Technology (FRCFT), Agriculture and Food Science Centre , University College Dublin, National University of Ireland , Belfield, Dublin 4 , Ireland.


Lycopene is a biologically active phytochemical reported in fruit. Conventional techniques such as high performance liquid chromatography (HPLC) and thin layer chromatography (TLC) have been in existence for measuring lycopene in fruit, but these methods are destructive with relative accuracy and speed. Other novel spectroscopic and imaging approaches, which are more reliable and fast, have recently been developed to investigate complex components such as lycopene, total soluble solids, etc. in fruit. The current review attempts to highlight the potential of both conventional and novel techniques in evaluating lycopene contents of fruit. The novel techniques include both spectroscopic methods such as near infrared spectroscopy and Raman spectroscopy and spectral imaging approaches such as multispectral imaging, hyperspectral imaging and Raman imaging. The principles of these techniques are summarized, their detailed applications are discussed, and future trends are also presented. Both traditional and novel techniques highlighted in the current review can be used for assessing the distribution and concentration of lycopene in various fruit. Although novel spectroscopic and spectral imaging approaches may in the near future replace conventional methods, because conventional methods are typically often offline, destructive and time-consuming, which also require the use of chemicals.


Lycopene; fruit; imaging techniques; spectroscopic techniques

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