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Nutrients. 2018 Dec 21;11(1). pii: E20. doi: 10.3390/nu11010020.

Cordyceps militaris Grown on Germinated Soybean Suppresses KRAS-Driven Colorectal Cancer by Inhibiting the RAS/ERK Pathway.

Author information

1
Department of Food Biotechnology, Division of Bioindustry, Silla University, Busan 46958, Korea. ssk4623@naver.com.
2
Department of Cogno-Mechatronics Engineeering, College of Nanoscience & Nanotechnology, Pusan National University, Busan 46241, Korea. ssk4623@naver.com.
3
Department of Food Biotechnology, Division of Bioindustry, Silla University, Busan 46958, Korea. jisu632@naver.com.
4
Department of Medical Science, School of Medicine, Pusan National University, Yangsan 50612, Korea. jisu632@naver.com.
5
Department of Electronics & IT Media Engineering, Seoul National University of Science & Technology, Seoul 01811, Korea. mkim21@gmail.com.
6
Department of Cogno-Mechatronics Engineeering, College of Nanoscience & Nanotechnology, Pusan National University, Busan 46241, Korea. nanohan@pusan.ac.kr.
7
Department of Food Biotechnology, Gachon University, Kyungji-do 13120, Korea. nimpi79@daum.net.
8
Department of Food Biotechnology, Division of Bioindustry, Silla University, Busan 46958, Korea. songmj@gmail.com.

Abstract

Cordyceps militaris is a commonly used medicinal mushroom containing various therapeutic effects such as anti-inflammatory, anti-allergic, and anti-cancer activities. This study examined whether Cordyceps militaris on germinated soybeans (GSC) has a suppressive effect on a v-ki-ras2 Kirsten rat sarcoma viral oncogene homolog (KRAS)-driven colorectal cancer which is notorious for its un-druggable features and the ineffectiveness of conventional therapies against it. GSC extract was prepared and its proximate composition and amino acids were analyzed. The suppressive effects were investigated with the KRAS-driven colorectal cancer cell-line, SW480. SW480 proliferation, clonogenic potential, apoptosis, and the RAS/extracellular signal-regulated kinase (ERK) pathway under the GSC treatment were analyzed by 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS) assay, flow cytometry, and Western blot, respectively. An in vivo experiment with the SW480 xenograft mouse model was performed. As a result, GSC suppressed cell proliferation by inducing the apoptosis of KRAS-driven colorectal cancer cells and inhibited clonogenic capabilities. The decrease of KRAS and ERK phosphorylation was detected by Western blot. Tumor growth was significantly suppressed when GSC was introduced to the tumor-xenograft mouse model. In conclusion, GSC suppressed KRAS-driven colorectal cancer growth both in vitro and in vivo, and can be used as an alternative or simultaneous approach in colorectal cancer therapy.

KEYWORDS:

Cordyceps militaris; KRAS; SW480; colorectal cancer

PMID:
30577618
PMCID:
PMC6356671
DOI:
10.3390/nu11010020
[Indexed for MEDLINE]
Free PMC Article

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