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Int J Mol Sci. 2018 Dec 20;20(1). pii: E10. doi: 10.3390/ijms20010010.

Glycopolymer Grafted Silica Gel as Chromatographic Packing Materials.

Author information

1
School of Chemical Engineering, Xiangtan University, Xiangtan 411105, China. Magq@smail.xtu.edu.cn.
2
CAS Key Laboratory of Bio-Based Materials, Qingdao Institute of Bioenergy and Bioprocess Technology (QIBEBT), Chinese Academy of Sciences (CAS), Qingdao 266101, China. Magq@smail.xtu.edu.cn.
3
CAS Key Laboratory of Bio-Based Materials, Qingdao Institute of Bioenergy and Bioprocess Technology (QIBEBT), Chinese Academy of Sciences (CAS), Qingdao 266101, China. luoxt@qibebt.ac.cn.
4
University of Chinese Academy of Sciences, Shijingshan District, Beijing 100049, China. luoxt@qibebt.ac.cn.
5
CAS Key Laboratory of Bio-Based Materials, Qingdao Institute of Bioenergy and Bioprocess Technology (QIBEBT), Chinese Academy of Sciences (CAS), Qingdao 266101, China. sunxt@dicp.ac.cn.
6
School of Chemical Engineering, Xiangtan University, Xiangtan 411105, China. wangweiyan@xtu.edu.cn.
7
CAS Key Laboratory of Bio-Based Materials, Qingdao Institute of Bioenergy and Bioprocess Technology (QIBEBT), Chinese Academy of Sciences (CAS), Qingdao 266101, China. shouqh@qibebt.ac.cn.
8
CAS Key Laboratory of Bio-Based Materials, Qingdao Institute of Bioenergy and Bioprocess Technology (QIBEBT), Chinese Academy of Sciences (CAS), Qingdao 266101, China. liangxf@qibebt.ac.cn.
9
CAS Key Laboratory of Bio-Based Materials, Qingdao Institute of Bioenergy and Bioprocess Technology (QIBEBT), Chinese Academy of Sciences (CAS), Qingdao 266101, China. hzliu@ipe.ac.cn.

Abstract

The modification of the surface of silica gel to prepare hydrophilic chromatographic fillers has recently become a research interest. Most researchers have grafted natural sugar-containing polymers onto chromatographic surfaces. The disadvantage of this approach is that the packing structure is singular and the application scope is limited. In this paper, we explore the innovative technique of grafting a sugar-containing polymer, 2-gluconamidoethyl methacrylamide (GAEMA), onto the surface of silica gel by atom transfer radical polymerization (ATRP). The SiO₂-g-GAEMA with ATRP reaction time was characterized by Fourier infrared analysis, Thermogravimetric analysis (TGA), and elemental analysis. As the reaction time lengthened, the amount of GAEMA grafted on the surface of the silica gel gradually increased. The GAEMA is rich in amide bonds and hydroxyl groups and is a typical hydrophilic chromatography filler. Finally, SiO₂-g-GAEMA (reaction time = 24 h) was chosen as the stationary phase of the chromatographic packing and evaluated with four polar compounds (uracil, cytosine, guanosine, and cytidine). Compared with unmodified silica gel, modified silica gel produces sharper peaks and better separation efficiency. This novel packing material may have a potential for application with highly isomerized sugar mixtures.

KEYWORDS:

ATRP; SiO2-g-GAEMA; glycopolymers; packing materials

PMID:
30577498
PMCID:
PMC6337448
DOI:
10.3390/ijms20010010
[Indexed for MEDLINE]
Free PMC Article

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