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Mol Immunol. 2019 Feb;106:46-52. doi: 10.1016/j.molimm.2018.12.004. Epub 2018 Dec 18.

Design, biological activity and signaling pathway of bovine consensus omega interferon expressed in Pichia pastoris.

Author information

1
Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Jilin University, Jilin, Changchun, 130062, PR China; Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Northeast Agricultural University, Harbin, Heilongjiang, 150030, PR China.
2
Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Northeast Agricultural University, Harbin, Heilongjiang, 150030, PR China; Animal Disease Prevention and Control Center of Heilongjiang Province, Harbin, Heilongjiang, 150069, PR China.
3
Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Northeast Agricultural University, Harbin, Heilongjiang, 150030, PR China.
4
Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Northeast Agricultural University, Harbin, Heilongjiang, 150030, PR China. Electronic address: jwwang@neau.edu.cn.
5
Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Jilin University, Jilin, Changchun, 130062, PR China; Jiangsu Co-Innovation Center for the Prevention and Control of Important Animal Infectious Disease and Zoonose,Yangzhou University,Yangzhou, 225009,PR China. Electronic address: hanwy@jlu.edu.cn.

Abstract

The bovine IFN-ω (BoIFN-ω) multigene family is located on chromosome 8, which has 14 potential functional genes and 10 pseudogenes. After aligning 14 BoIFN-ω subtypes and assigning the most frequently occurring amino acids in each position, one artificial consensus BoIFN-ω (CoBoIFN-ω) gene was designed, optimized and synthesized. Then, CoBoIFN-ω was expressed in Pichia pastoris, which was demonstrated to have 3.94-fold and 14.3-fold higher antiviral activity against VSV on MDBK cells than that of BoIFN-ω24 and BoIFN-ω3, respectively. Besides this, CoBoIFN-ω was confirmed to have antiviral activity against VSV on BL, BT, PK-15 cells, and against BEV, BHV-1, BPIV3 on MDBK cells. Additionally, CoBoIFN-ω could bind with bovine type I IFN receptors, and then activate the promoters of NF-κB, ISRE and BoIFN-β, and induce the transcription of ISGs and expression of Mx1 and NF-κB p65, which suggested CoBoIFN-ω exerts antiviral activity via activation of the JAK-STAT signaling pathway. Overall, this research on CoBoIFN-ω not only extends and improves consensus IFN research, but also reveals that CoBoIFN-ω has the potential to be used in the therapy of bovine viral diseases.

KEYWORDS:

Biological activity; Bovine IFN-ω; Consensus interferon; Signaling pathway

PMID:
30576951
DOI:
10.1016/j.molimm.2018.12.004
[Indexed for MEDLINE]

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