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Am J Physiol Endocrinol Metab. 2018 Dec 21. doi: 10.1152/ajpendo.00244.2018. [Epub ahead of print]

Increased circulating miR-370-3p regulates Steroidogenic Factor 1 in Endometriosis.

Author information

1
Obstetrics, Gynecology & Reproductive Sciences, Yale School of Medicine, China.
2
Internal Medicine (Endocrinology), Yale University School of Medicine, United States.
3
Obstetrics and Gynecology and Reproductive Sciences, Yale University School of Medicine, United States.

Abstract

Endometriosis is a gynecological disease among reproductive-aged women caused by the growth of endometrial tissue outside the uterus. Altered expression of genes and microRNAs has been reported in endometriosis. Steroidogenic factor 1 (SF-1), an essential transcriptional regulator of multiple genes involved in estrogen biosynthesis; is aberrantly increased and plays an important role in the pathogenesis of endometriosis. Here, we show the expression of SF-1 in endometriosis is regulated by microRNA miR-370-3p. Sera and tissue were collected from surgically diagnosed twenty women with endometriosis and twenty six women without endometriosis. We found that miR-370-3p levels were decreased in the serum of patients with endometriosis while SF-1 mRNA levels were inversely upregulated in endometriotic lesions compared to respective controls. Transfection of primary endometriotic cells with miR-370-3p mimic or inhibitor resulted in the altered expression of SF-1 and SF-1 downstream genes, StAR and CYP19A1. Overexpression of miR-370-3p inhibited cell proliferation and induced apoptosis in endometriotic cells. This study reveals that miR-370-3p functions as a negative regulator of SF-1 and cell proliferation in endometriotic cells. This study provides a novel therapeutic strategy for controlling SF-1 in endometriosis.

KEYWORDS:

Endometriosis; SF-1; apoptosis; cell proliferation; miR-370-3p

PMID:
30576245
DOI:
10.1152/ajpendo.00244.2018

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