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Nat Commun. 2018 Dec 21;9(1):5455. doi: 10.1038/s41467-018-07801-x.

A miR-150/TET3 pathway regulates the generation of mouse and human non-classical monocyte subset.

Author information

1
INSERM U1170, Gustave Roussy Cancer Center, 94805, Villejuif, France. dorothee.selimoglubuet@gustaveroussy.fr.
2
INSERM U1170, Gustave Roussy Cancer Center, 94805, Villejuif, France.
3
Université Paris-Sud, Faculté de Médecine, 94270, Le Kremlin-Bicêtre, France.
4
INSERM US23, CNRS UMS 3655, Gustave Roussy Cancer Center, 94805, Villejuif, France.
5
Laboratoire d'Hématologie, Centre Hospitalier Régional Universitaire, 29200, Brest, France.
6
Laboratoire d'Hématologie, Centre Hospitalier Régional Universitaire, 44000, Nantes, France.
7
Département d'Hématologie, Hôpital Saint-Louis, Assistance Publique-Hôpitaux de Paris, 75010, Paris, France.
8
Département d'Immuno-Hématologie, Institut Cochin, 75014, Paris, France.
9
Université Paris Diderot, 75004, Paris, France.
10
Département d'Hématologie, Gustave Roussy Cancer Center, 94805, Villejuif, France.
11
Département d'Hématologie et d'Immunologie Biologiques, Hôpital Henri-Mondor, 94010, Créteil, France.
12
INSERM U1170, Gustave Roussy Cancer Center, 94805, Villejuif, France. eric.solary@gustaveroussy.fr.
13
Université Paris-Sud, Faculté de Médecine, 94270, Le Kremlin-Bicêtre, France. eric.solary@gustaveroussy.fr.
14
Département d'Hématologie, Gustave Roussy Cancer Center, 94805, Villejuif, France. eric.solary@gustaveroussy.fr.

Abstract

Non-classical monocyte subsets may derive from classical monocyte differentiation and the proportion of each subset is tightly controlled. Deregulation of this repartition is observed in diverse human diseases, including chronic myelomonocytic leukemia (CMML) in which non-classical monocyte numbers are significantly decreased relative to healthy controls. Here, we identify a down-regulation of hsa-miR-150 through methylation of a lineage-specific promoter in CMML monocytes. Mir150 knock-out mice demonstrate a cell-autonomous defect in non-classical monocytes. Our pulldown experiments point to Ten-Eleven-Translocation-3 (TET3) mRNA as a hsa-miR-150 target in classical human monocytes. We show that Tet3 knockout mice generate an increased number of non-classical monocytes. Our results identify the miR-150/TET3 axis as being involved in the generation of non-classical monocytes.

PMID:
30575719
PMCID:
PMC6303340
DOI:
10.1038/s41467-018-07801-x
[Indexed for MEDLINE]
Free PMC Article

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