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Indian J Ophthalmol. 2019 Jan;67(1):38-41. doi: 10.4103/ijo.IJO_568_18.

Dopamine levels in human tear fluid.

Author information

1
GROW Research Laboratory, Narayana Nethralaya Foundation, Bengaluru, Karnataka, India.
2
Narayana Nethralaya Eye Hospital, Bengaluru, Karnataka, India.

Abstract

Purpose:

To determine the levels of dopamine in tear fluid and demonstrate the use of tear fluid as a non-invasive source for dopamine measurements in humans.

Methods:

The study cohort included 30 clinically healthy individuals without any pre-existing ocular or systemic conditions. Matched tear fluid (using Schirmer's strips and capillary tubes) and plasma were collected from the subjects. Dopamine levels were evaluated using direct competitive chemiluminescent enzyme-linked immunosorbent assay (ELISA), dopamine kit (Cloud Clone Corp, TX, USA).

Results:

Significantly higher dopamine levels were found in the tear fluid compared to plasma in the study subjects. The level of dopamine was 97.2 ± 11.80 pg/ml (mean ± SEM), 279 ± 14.8 pg/ml (mean ± SEM), and 470.4 ± 37.64 pg/ml (mean ± SEM) in the plasma and in the tears collected using Schirmer's strips and capillary tubes, respectively.

Conclusion:

Dopamine was detectable in all the tear fluid samples tested and was also found to be at a higher concentration than in plasma samples. Tear fluid can be used as a non-invasive sample source to monitor dopamine levels.

KEYWORDS:

Dopamine; ELISA; plasma; tear fluid

PMID:
30574889
DOI:
10.4103/ijo.IJO_568_18
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