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Sci Rep. 2018 Dec 20;8(1):18008. doi: 10.1038/s41598-018-36195-5.

Molecular comparison of Neanderthal and Modern Human adenylosuccinate lyase.

Author information

1
Structural Biology Group, European Synchrotron Radiation Facility, CS 40220, F-38043, Grenoble, France.
2
Max Planck Institute for Evolutionary Anthropology, D-04103, Leipzig, Germany.
3
The Francis Crick Institute, London, NW1 1AT, United Kingdom.
4
Structural Biology Group, European Synchrotron Radiation Facility, CS 40220, F-38043, Grenoble, France. christoph.mueller_dieckmann@esrf.fr.

Abstract

The availability of genomic data from extinct homini such as Neanderthals has caused a revolution in palaeontology allowing the identification of modern human-specific protein substitutions. Currently, little is known as to how these substitutions alter the proteins on a molecular level. Here, we investigate adenylosuccinate lyase, a conserved enzyme involved in purine metabolism for which several substitutions in the modern human protein (hADSL) have been described to affect intelligence and behaviour. During evolution, modern humans acquired a specific substitution (Ala429Val) in ADSL distinguishing it from the ancestral variant present in Neanderthals (nADSL). We show here that despite this conservative substitution being solvent exposed and located distant from the active site, there is a difference in thermal stability, but not enzymology or ligand binding between nADSL and hADSL. Substitutions near residue 429 which do not profoundly affect enzymology were previously reported to cause neurological symptoms in humans. This study also reveals that ADSL undergoes conformational changes during catalysis which, together with the crystal structure of a hitherto undetermined product bound conformation, explains the molecular origin of disease for several modern human ADSL mutants.

PMID:
30573755
PMCID:
PMC6301967
DOI:
10.1038/s41598-018-36195-5
[Indexed for MEDLINE]
Free PMC Article

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