Format

Send to

Choose Destination
Sci Rep. 2018 Dec 20;8(1):18007. doi: 10.1038/s41598-018-36703-7.

Induction of Human T-cell and Cytokine Responses Following Vaccination with a Novel Influenza Vaccine.

Author information

1
Institute of Molecular and Cell Biology, 61 Biopolis Drive, Proteos Bldg, Singapore, 138673, Singapore.
2
A*STAR Program in Translational Research on Infectious Disease, Agency for Science, Technology and Research, Singapore, Singapore.
3
Genome Institute of Singapore, Agency for Science, Technology and Research, Singapore, Singapore.
4
Singapore Immunology Network (SIgN), 8A Biomedical Grove, Immunos Bldg, Singapore, 138648, Singapore.
5
SingHealth Investigational Medicine Unit, Singapore General Hospital, Block 7, Outram Rd., Singapore, 169610, Singapore.
6
Singapore General Hospital, 20 College Road, Singapore, 169856, Singapore.
7
Clinical Trials Research Unit, Changi General Hospital, 2 Simei St. 3, Singapore, 529889, Singapore.
8
National University of Singapore, Department of Medicine, 1E Kent Ridge Road, Singapore, 119228, Singapore.
9
Cytos Biotechnology AG, Wagistr. 25, Zürich-Schlieren, CH-8952, Switzerland.
10
Inselspital, RIA, Immunologie, Sahlihaus 2, CH-3010, Bern, Switzerland.
11
Duke-NUS Medical School, Program in Emerging Infectious Diseases, 8 College Rd., Singapore, 169857, Singapore.
12
DSO National Laboratories, Bio-Defense Therapeutics Lab, 27 Medical Drive, Singapore, 117510, Singapore.
13
D3 (Drug Discovery and Development), 31 Biopolis Way, #01-02a Nanos, Singapore, 138669, Singapore.
14
Institute of Molecular and Cell Biology, 61 Biopolis Drive, Proteos Bldg, Singapore, 138673, Singapore. jeconnolly@imcb.a-star.edu.sg.
15
A*STAR Program in Translational Research on Infectious Disease, Agency for Science, Technology and Research, Singapore, Singapore. jeconnolly@imcb.a-star.edu.sg.
16
Institute of Biomedical Studies, Baylor University, Waco, Texas, 76712, USA. jeconnolly@imcb.a-star.edu.sg.

Abstract

Cell mediated immunity plays a vital role in defense against influenza infection in humans. Less is known about the role of vaccine-induced cell mediated immunity and the cytokine responses elicited. We measured CD4+ and CD8+ T-cell reactivity in human subjects following vaccination with licensed trivalent influenza vaccine and a novel virus-like particle based vaccine. We detected influenza-specific CD4+ T-cell responses following vaccination with the licensed trivalent influenza vaccine and found that these correlated with antibody measurements. Administration of the novel virus-like particle based vaccine elicited influenza-specific CD4+ and CD8+ T-cell responses and the induction of the cytokines IFN-γ, IL-17A, IL17F, IL-5, IL-13, IL-9, IL-10 and IL-21. Pre-existing cytokine responses influenced the profile of the cytokine response elicited by vaccination. In a subset of individuals the VLP vaccine changed pre-vaccination production of type 2 cytokines such as IL-5 and IL-13 to a post-vaccination type 1 cytokine signature characterized by IFN-γ. A transcriptional signature to vaccination was found to correlate with antibody titer, IFN-γ production by T-cells and expression of a putative RNA helicase, DDX17, on the surface of immune cells.

Supplemental Content

Full text links

Icon for Nature Publishing Group Icon for PubMed Central
Loading ...
Support Center