Format

Send to

Choose Destination
Science. 2018 Dec 21;362(6421):1423-1428. doi: 10.1126/science.aat1839.

ATP-dependent force generation and membrane scission by ESCRT-III and Vps4.

Schöneberg J1,2,3, Pavlin MR#2,4, Yan S#1,2, Righini M5, Lee IH1,2, Carlson LA1,2, Bahrami AH3, Goldman DH2,6,5, Ren X1,2, Hummer G3,7, Bustamante C8,2,4,6,5,9,10, Hurley JH8,2,4,10.

Author information

1
Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA 94720, USA.
2
California Institute for Quantitative Biosciences, University of California, Berkeley, Berkeley, CA 94720, USA.
3
Department of Theoretical Biophysics, Max Planck Institute of Biophysics, 60438 Frankfurt am Main, Germany.
4
Graduate Group in Biophysics, University of California, Berkeley, Berkeley, CA 94720, USA.
5
Department of Chemistry, University of California, Berkeley, Berkeley, CA 94720, USA.
6
Howard Hughes Medical Institute, University of California, Berkeley, Berkeley, CA 94720, USA.
7
Institute of Biophysics, Goethe University, Frankfurt/M, Germany.
8
Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA 94720, USA. jimhurley@berkeley.edu carlosb@berkeley.edu.
9
Department of Physics, University of California, Berkeley, Berkeley, CA 94720, USA.
10
Molecular Biophysics and Integrated Bioimaging Division, Lawrence Berkeley National Laboratory, Berkeley, CA 94720, USA.
#
Contributed equally

Abstract

The endosomal sorting complexes required for transport (ESCRTs) catalyze reverse-topology scission from the inner face of membrane necks in HIV budding, multivesicular endosome biogenesis, cytokinesis, and other pathways. We encapsulated ESCRT-III subunits Snf7, Vps24, and Vps2 and the AAA+ ATPase (adenosine triphosphatase) Vps4 in giant vesicles from which membrane nanotubes reflecting the correct topology of scission could be pulled. Upon ATP release by photo-uncaging, this system generated forces within the nanotubes that led to membrane scission in a manner dependent upon Vps4 catalytic activity and Vps4 coupling to the ESCRT-III proteins. Imaging of scission revealed Snf7 and Vps4 puncta within nanotubes whose presence followed ATP release, correlated with force generation and nanotube constriction, and preceded scission. These observations directly verify long-standing predictions that ATP-hydrolyzing assemblies of ESCRT-III and Vps4 sever membranes.

PMID:
30573630
PMCID:
PMC6309985
[Available on 2019-06-21]
DOI:
10.1126/science.aat1839

Supplemental Content

Full text links

Icon for HighWire
Loading ...
Support Center