Format

Send to

Choose Destination
Pharmaceutics. 2018 Dec 18;10(4). pii: E286. doi: 10.3390/pharmaceutics10040286.

Autologous Red Blood Cell Delivery of Betamethasone Phosphate Sodium for Long Anti-Inflammation.

Author information

1
School of Pharmacy, Shanghai Jiao Tong University, Shanghai 200240, China. xiumeizhang@sjtu.edu.cn.
2
School of Pharmacy, Shanghai Jiao Tong University, Shanghai 200240, China. mfqiu@sjtu.edu.cn.
3
School of Pharmacy, Shanghai Jiao Tong University, Shanghai 200240, China. gpcedu@sjtu.edu.cn.
4
School of Pharmacy, Shanghai Jiao Tong University, Shanghai 200240, China. lym-0517@sjtu.edu.cn.
5
School of Pharmacy, Shanghai Jiao Tong University, Shanghai 200240, China. engles@sjtu.edu.cn.
6
School of Pharmacy, Shanghai Jiao Tong University, Shanghai 200240, China. jingsu@sjtu.edu.cn.

Abstract

Although glucocorticoids are highly effective in treating various types of inflammation such as skin disease, rheumatic disease, and allergic disease, their application have been seriously limited for their high incidence of side effects, particularly in long term treatment. To improve efficacy and reduce side effects, we encapsulated betamethasone phosphate (BSP) into biocompatible red blood cells (RBCs) and explored its long acting-effect. BSP was loaded into rat autologous erythrocytes by hypotonic preswelling method, and the loading amount was about 2.5 mg/mL cells. In vitro, BSP loaded RBCs (BSP-RBCs) presented similar morphology, osmotic fragility to native RBCs (NRBCs). After the loading process, the loaded cells can maintain around 70% of Na⁺/K⁺-ATPase activity of natural cells. In vivo, a series of tests including survival, pharmacokinetics, and anti-inflammatory effect were carried out to examine the long-acting effect of BSP-RBCs. The results shown that the loaded cells could circulate in plasma for over nine days, the release of BSP can last for over seven days and the anti-inflammatory effect can still be observed on day 5 after injection. Totally, BSP-loaded autologous erythrocytes seem to be a promising sustained releasing delivery system with long anti-inflammatory effect.

KEYWORDS:

betamethasone phosphate; drug delivery system; long anti-inflammation; red blood cells

Supplemental Content

Full text links

Icon for Multidisciplinary Digital Publishing Institute (MDPI) Icon for PubMed Central
Loading ...
Support Center