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J Cell Mol Med. 2019 Mar;23(3):1865-1872. doi: 10.1111/jcmm.14087. Epub 2018 Dec 19.

The long non-coding RNA-ROR promotes osteosarcoma progression by targeting miR-206.

Author information

1
Ultrasonographic Department, China-Japan Union Hospital of Jilin University, Changchun, P.R. China.
2
Department of Orthopaedic Surgery, China-Japan Union Hospital of Jilin University, Changchun, P.R. China.
3
Department of Thoracic Surgery, The First Hospital of Jilin University, Changchun, P.R. China.

Abstract

The long intergenic non-protein coding RNA regulator of reprogramming (lncRNA-ROR) has been reported to play crucial regulatory roles in the pathogenesis and progression of multiple cancers. However, whether ROR is associated with the initiation and development of osteosarcoma (OS) remains unclear. Here, we found that ROR expression level was significantly up-regulated in OS tissue samples compared to adjacent normal tissues, and the elevated ROR was closely correlated with advanced tumour-node-metastasis (TNM) stage and lymph node metastasis and poor overall survival rate. Functional assays showed that ROR knockdown suppressed the OS cell proliferation, colony formation, migration and invasion in vitro, and retarded tumour growth in vivo. In addition, miR-206 was verified to be a target miRNA of ROR using bioinformatics online program and luciferase report assay. miR-206 inhibition partially rescued the inhibitory effects on OS cells induced by ROR knockdown. In conclusion, these results suggested that ROR function as an oncogene in OS by sponging miR-206 and might be a potential therapeutic target for patients with OS.

KEYWORDS:

ROR; invasion; lncRNA; miR-206; osteosarcoma; proliferation

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