Format

Send to

Choose Destination
Am J Clin Pathol. 2019 Mar 1;151(4):416-423. doi: 10.1093/ajcp/aqy160.

Application of Fluorescence In Situ Hybridization on Cerebrospinal Fluid Cytospins for the Detection of Residual Leukemic Cells in Patients With Childhood Acute Lymphoblastic Leukemia.

Author information

1
Department of Laboratory Medicine, Seoul National University Bundang Hospital, Seongnam, Korea.
2
Department of Laboratory Medicine, Seoul National University College of Medicine, Seoul, Korea.
3
Department of Laboratory Medicine, Chungbuk National University Hospital, Cheongju, Korea.
4
Green Cross Reference Laboratory, Yong-In, Gyeonggi-do, South Korea.
5
Cancer Research Institute, Seoul National University College of Medicine, Seoul, Korea.
6
Department of Pediatrics, Seoul National University College of Medicine, Seoul, Korea.
7
Department of Laboratory Medicine, Korea Cancer Center Hospital, Korea Institute of Radiological and Medical Sciences, Seoul, Korea.

Abstract

OBJECTIVES:

Diagnosis of central nervous system involvement in acute lymphoblastic leukemia (ALL) requires morphologic expertise; therefore, we evaluated interphase fluorescence in situ hybridization (iFISH) of cerebrospinal fluid (CSF) cytospin preparations as a potential complementary test.

METHODS:

Twenty-three CSF cytospin specimens from 13 pediatric patients with ALL were included. iFISH probes detecting BCR-ABL1, ETV6-RUNX1, and KMT2A rearrangement and CDKN2A deletion, which were present at initial diagnosis, were used on follow-up CSF cytospin specimens and were compared with cytology.

RESULTS:

Seventeen (73.9%) follow-up specimens showed concordant results between iFISH and cytology. Two (8.7%) samples with discordant results were positive by iFISH but not by cytology; one (4.3%) was positive only by cytology. In the remaining three (13.0%) specimens, too few cells were available for cytology, whereas iFISH interpretation was possible.

CONCLUSIONS:

iFISH of CSF cytospin preparations improves malignant cell detection in pediatric ALL.

KEYWORDS:

Acute lymphoblastic leukemia; Cerebrospinal fluid; Cytospin; Fluorescence in situ hybridization

PMID:
30561492
DOI:
10.1093/ajcp/aqy160

Supplemental Content

Full text links

Icon for Silverchair Information Systems
Loading ...
Support Center