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Front Microbiol. 2018 Nov 30;9:2833. doi: 10.3389/fmicb.2018.02833. eCollection 2018.

A mcr-1-Carrying Conjugative IncX4 Plasmid in Colistin-Resistant Escherichia coli ST278 Strain Isolated From Dairy Cow Feces in Shanghai, China.

Bai F1, Li X2, Niu B1, Zhang Z1, Malakar PK1, Liu H1,3,4,5, Pan Y1,3,5, Zhao Y1,3,5.

Author information

1
College of Food Science and Technology, Shanghai Ocean University, Shanghai, China.
2
State Key Laboratory of Microbial Metabolism, Joint International Laboratory of Metabolic and Developmental Sciences, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai, China.
3
Laboratory of Quality & Safety Risk Assessment for Aquatic Products on Storage and Preservation (Shanghai), Ministry of Agriculture, Shanghai, China.
4
Engineering Research Center of Food Thermal-processing Technology, Shanghai Ocean University, Shanghai, China.
5
Shanghai Engineering Research Center of Aquatic-Product Processing & Preservation, Shanghai, China.

Abstract

Enterobacteriaceae, including Escherichia coli, has been shown to acquire the colistin resistance gene mcr-1. A strain of E. coli, EC11, which is resistant to colistin, polymyxin B and trimethoprim-sulfamethoxazole, was isolated in 2016 from the feces of a dairy cow in Shanghai, China. Strain EC11 identifies with sequence type ST278 and is susceptible to 19 frequently used antibiotics. Whole genome sequencing of strain EC11 showed that this strain contains a 31-kb resistance plasmid, pEC11b, which belongs to the IncX4 group. The mcr-1 gene was shown to be inserted into a 2.6-kb mcr-1-pap2 cassette of pEC11b. Plasmid pEC11b also contained putative conjugal transfer components, including an oriT-like region, relaxase, type IV coupling protein, and type IV secretion system. We were successful in transferring pEC11b to E. coli C600 with an average transconjugation efficiency of 4.6 × 10-5. Additionally, a MLST-based analysis comparing EC11 and other reported mcr-positive E. coli populations showed high genotypic diversity. The discovery of the E. coli strain EC11 with resistance to colistin in Shanghai emphasizes the importance of vigilance in detecting new threats like mcr genes to public health. Detection of mcr genes helps in tracking, slowing, and responding to the emergence of antibiotic resistance in Chinese livestock farming.

KEYWORDS:

Escherichia coli; IncX4 plasmid; colistin resistance; mcr-1; whole genome sequence

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