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Nat Methods. 2019 Jan;16(1):59-62. doi: 10.1038/s41592-018-0259-9. Epub 2018 Dec 17.

Simultaneous multiplexed amplicon sequencing and transcriptome profiling in single cells.

Author information

1
Meinig School of Biomedical Engineering, Cornell University, Ithaca, NY, USA.
2
Baker Institute for Animal Health, College of Veterinary Medicine, Cornell University, Ithaca, NY, USA.
3
Meinig School of Biomedical Engineering, Cornell University, Ithaca, NY, USA. vlaminck@cornell.edu.

Abstract

We describe droplet-assisted RNA targeting by single-cell sequencing (DART-seq), a versatile technology that enables multiplexed amplicon sequencing and transcriptome profiling in single cells. We applied DART-seq to simultaneously characterize the non-A-tailed transcripts of a segmented dsRNA virus and the transcriptome of the infected cell. In addition, we used DART-seq to simultaneously determine the natively paired, variable region heavy and light chain amplicons and the transcriptome of B lymphocytes.

PMID:
30559431
PMCID:
PMC6378878
DOI:
10.1038/s41592-018-0259-9
[Indexed for MEDLINE]
Free PMC Article

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