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Exp Cell Res. 2019 Feb 1;375(1):31-41. doi: 10.1016/j.yexcr.2018.12.007. Epub 2018 Dec 15.

Pluripotency markers in tissue and cultivated cells in vitro of different regions of human amniotic epithelium.

Author information

1
Instituto Nacional de Perinatología, Departamento de Fisiología y Desarrollo Celular, Ciudad de México, Mexico.
2
Instituto Nacional de Perinatología, Departamento de Immuno-Bioquímica, Ciudad de México, Mexico.
3
Universidad Nacional Autónoma de México, Departamento de Neurobiología Conductual y Cognitiva, Instituto de Neurobiología, Querétaro, Mexico.
4
Centro de Investigación y Asistencia en Tecnología y Diseño del Estado de Jalisco, Unidad de Biotecnología Médica y Farmacéutica, Guadalajara, Jalisco, Mexico.
5
Universidad Nacional Autónoma de México, Unidad de Secuenciación Masiva y Bioinformática, Instituto de Biotecnología, Cuernavaca, Mexico.
6
Universidad Nacional Autónoma de México, Departamento de Biología Celular y Fisiología, Instituto de Investigaciones Biomédicas, Ciudad de México, Mexico.
7
Instituto Nacional de Perinatología, Departamento de Fisiología y Desarrollo Celular, Ciudad de México, Mexico. Electronic address: nfdiaz00@yahoo.com.mx.

Abstract

Studies have described the presence of pluripotent markers in vivo and in vitro in human amnion. However, the amnion can be divided into reflected, placental and umbilical regions that are anatomically and functionally heterogeneous. Here, we evaluated the expression of pluripotency markers in tissue and cultivated cells in vitro of different regions of human amnion. To this end, we determined the presence of the core pluripotency factors OCT-4, NANOG and SOX-2 by immunofluorescence and RT-PCR and also performed transcriptome analysis of the different regions of amnion tissue. We identified the mRNA and protein of the pluripotency factors in the different regions of human amnion tissue. However, the OCT-4 and NANOG immunolocalization was cytoplasmic, whereas SOX-2 immunolocalization was nuclear regardless of the region analyzed. Moreover, we found three subpopulations of cells in the in vitro cultures of reflected and placental amnion: cells with immunostaining only in the nucleus, only in the cytoplasm, or in both compartments. Yet no statistically significant differences were found between the reflected and placental amnion. These results suggest a homogeneous distribution of the pluripotency transcription factors of the different regions of human amnion to isolate stem cells that can be used in regenerative medicine.

KEYWORDS:

Amnion; Epiblast; Pluripotency

PMID:
30557557
DOI:
10.1016/j.yexcr.2018.12.007
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