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Biosens Bioelectron. 2019 Feb 1;126:767-772. doi: 10.1016/j.bios.2018.11.044. Epub 2018 Dec 6.

Simultaneous detection of three biomarkers related to acute myocardial infarction based on immunosensing biochip.

Author information

1
Jiangsu Engineering Laboratory of Smart Carbon-Rich Materials and Device, School of Chemistry and Chemical Engineering, Southeast University, Nanjing 211189, China.
2
Department of Chemistry, Liaocheng University, Liaocheng, Shandong 252059, China.
3
Nanjing Second Hospital, China.
4
Jiangsu Engineering Laboratory of Smart Carbon-Rich Materials and Device, School of Chemistry and Chemical Engineering, Southeast University, Nanjing 211189, China. Electronic address: wuyafeng_1985@163.com.
5
Jiangsu Engineering Laboratory of Smart Carbon-Rich Materials and Device, School of Chemistry and Chemical Engineering, Southeast University, Nanjing 211189, China. Electronic address: liusq@seu.edu.cn.

Abstract

An immunosensing biochip for simultaneous detection of three biomarkers related to acute myocardial infarction (AMI) was developed based on anionic soybean peroxidase (SBP) functionalized nanoprobe and chemiluminescent imaging. The nanoprobes (Ab2-SiO2-SBP) were fabricated by co-immobilization of SBP and one of the detection polyclonal antibodies, anti-cardiac troponin I antigen (anti-cTnI), anti-creatine kinase-MB (anti-CK-MB) and anti-myoglobin (anti-Myo), on the silica nanoparticle surface. The detection sensitivity was enhanced since the large surface area of silica carriers increased the loading of SBP for per sandwiched immunoreaction. The immunosensing biochip designed as 3 × 8 wells array was constructed by simultaneously immobilizing three capture monoclonal antibodies on the same one microtiter well with 2 × 3 active spots. In the presence of target protein, the nanoprobes will be attached onto the spots with high specificity through the sandwiched immunoreactions, which triggered the chemiluminescence (CL) signals on each sensing site of the microtiter plates and allowed to CL imaging of three biomarkers in one well at the same time. Therefore, the proposed biochip was a promising convenient strategy for simultaneous detection of cTnI, CK-MB and Myo, which showed potential application for multianalyte determination in clinical diagnostics.

KEYWORDS:

Acute myocardial infarction; Chemiluminescence imaging; Immunosensing biochip; Nanoprobes; Simultaneous detection

PMID:
30554098
DOI:
10.1016/j.bios.2018.11.044
[Indexed for MEDLINE]

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