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J Pharm Biomed Anal. 2019 Feb 20;165:213-223. doi: 10.1016/j.jpba.2018.11.038. Epub 2018 Nov 17.

Study on anti-hyperuricemia effects and active ingredients of traditional Tibetan medicine TongFengTangSan (TFTS) by ultra-high-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry.

Author information

1
Jiangxi University of Traditional Chinese Medicine, No. 1688 Meiling Road, Nanchang, 330004, PR China.
2
Nanchang Insitute for Food and Drug Control, No. 299 Diezihu Road, Nanchang, 330038, PR China.
3
Jiangxi University of Traditional Chinese Medicine, No. 1688 Meiling Road, Nanchang, 330004, PR China. Electronic address: fengyulin2003@126.com.
4
Jiangxi University of Traditional Chinese Medicine, No. 1688 Meiling Road, Nanchang, 330004, PR China. Electronic address: Zwgchf98@foxmail.com.

Abstract

TongFengTangSan (TFTS), a traditional Tibetan medicine comprising of Tinospora sinensis (TS), Terminalia chebula Retz (TC) and Trogopterori faeces (TF), is used to treat joint diseases like gout, gout arthritis, swelling, pain etc. Despite the significant therapeutic effects of TFTS, its pharmacological components have not been analyzed so far. Therefore, the chemical composition of the effective part of TFTS was analyzed by ultra-high-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (UHPLC-Q-TOF-MS/MS). The results show that the ethanol extract (EE) of TFTS was more effective in reducing the serum uric acid (SUA) and XOD (Serum and Liver) levels in a hyperuricemic rats model compared to the TFTS raw powder (RP). UHPLC-Q-TOF-MS/MS identified a total of 106 compounds in the positive and negative ion mode, of which 87 were from TC, 13 from TS and 6 from TF. In addition, 106 compounds contained 57 tannins, 6 triterpenoids, 10 alkaloids, 7 flavonoids, 22 organic acids and 4 phenylpropanoids. The preliminary results indicate that the EE of TFTS includes the active anti hyperuricemic substances. The present study first investigated the efficacy and the active components of TFTS in hyperuricemic treatment, and further summarized the diagnostic ion and neutral loss patterns of MS/MS cracking of tannic compounds. These findings lay the foundation for the further study and clinical application of TFTS.

KEYWORDS:

Active ingredients; Anti-hyperuricemic effects; MS/MS cracking rule; TongFengTangSan; Traditional Tibetan medicine; UHPLC-Q-TOF-MS/MS

PMID:
30553111
DOI:
10.1016/j.jpba.2018.11.038

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