Two-dimensional porphyrin-based covalent organic framework: A novel platform for sensitive epidermal growth factor receptor and living cancer cell detection

A porphyrin-based covalent organic framework (denoted as p-COF) was synthesized by a simple oil-bath method and exploited as a novel sensing layer for immobilizing epidermal growth factor receptor (EGFR)-targeting aptamer strands to detect trace EGFR and living michigan cancer foundation-7 (MCF-7) cells for the first time. p-COF presented a nanosheet-like structure, large cavities, rich nitrogen-bearing groups, high electrochemical activity, excellent bioaffinity, low toxicity, and good stability in aqueous solution; the microstructural features of this material enabled strong immobilization of the aptamer strands. Interactions between the aptamer strands and EGFR significantly changed the electrochemical signals of the modified electrode due to the formation of an aptamer-EGFR complex. The p-COF-based aptasensor exhibited an extremely low detection limit (LOD) of 5.64 fg·mL^-1 obtained from differential pulse voltammetry and 7.54 fg·mL^-1 originated from electrochemical impedance spectroscopy with a broad linear detection range of 0.05-100 pg·mL^-1 of the EGFR concentration. When detecting living MCF-7 cells, the p-COF-based aptasensor showed an LOD of 61 cell·mL^-1 with a linear detection range of 500 × 10⁵ cell·mL^-1. The fabricated aptasensor exhibited high selectivity, good stability, reproducibility, acceptable recyclability, and favorable applicability in human serum samples. We believe that the developed p-COF-based aptasensor is a potential candidate for the sensitive detection of target cancer markers or living cells.