Format

Send to

Choose Destination
Sci Rep. 2018 Dec 14;8(1):17886. doi: 10.1038/s41598-018-36148-y.

Plasmodium falciparum gametocyte-infected erythrocytes do not adhere to human primary erythroblasts.

Neveu G1,2,3,4, Dupuy F1,2,3,4, Ladli M1,2,3,4, Barbieri D1,2,3,4, Naissant B1,2,3,4, Richard C1,2,3,4, Martins RM5, Lopez-Rubio JJ5, Bachmann A6, Verdier F1,2,3,4, Lavazec C7,8,9,10.

Author information

1
Inserm U1016, Institut Cochin, Paris, France.
2
Cnrs, UMR8104, Paris, France.
3
Université Paris Descartes, Sorbonne Paris Cité, Paris, France.
4
Laboratoire d'excellence GR-Ex, Paris, France.
5
UMR IRD 224-CNRS 5290-University Montpellier, MIVEGEC, Montpellier, France.
6
Bernhard Nocht Institute for Tropical Medicine, Hamburg, Germany.
7
Inserm U1016, Institut Cochin, Paris, France. catherine.lavazec@inserm.fr.
8
Cnrs, UMR8104, Paris, France. catherine.lavazec@inserm.fr.
9
Université Paris Descartes, Sorbonne Paris Cité, Paris, France. catherine.lavazec@inserm.fr.
10
Laboratoire d'excellence GR-Ex, Paris, France. catherine.lavazec@inserm.fr.

Abstract

Plasmodium falciparum gametocytes, the sexual stages responsible for malaria parasite transmission, develop in the human bone marrow parenchyma in proximity to the erythroblastic islands. Yet, mechanisms underlying gametocytes interactions with these islands are unknown. Here, we have investigated whether gametocyte-infected erythrocytes (GIE) adhere to erythroid precursors, and whether a putative adhesion may be mediated by a mechanism similar to the adhesion of erythrocytes infected with P. falciparum asexual stages to uninfected erythrocytes. Cell-cell adhesion assays with human primary erythroblasts or erythroid cell lines revealed that immature GIE do not specifically adhere to erythroid precursors. To determine whether adhesion may be dependent on binding of STEVOR proteins to Glycophorin C on the surface of erythroid cells, we used clonal lines and transgenic parasites that overexpress specific STEVOR proteins known to bind to Glycophorin C in asexual stages. Our results indicate that GIE overexpressing STEVOR do not specifically adhere to erythroblasts, in agreement with our observation that the STEVOR adhesive domain is not exposed at the surface of GIE.

Publication type, Grant support

Publication type

Grant support

Supplemental Content

Full text links

Icon for Nature Publishing Group Icon for PubMed Central
Loading ...
Support Center