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Appl Environ Microbiol. 2018 Dec 14. pii: AEM.02365-18. doi: 10.1128/AEM.02365-18. [Epub ahead of print]

Population genetics and characterization of Campylobacter jejuni isolates in western jackdaws and game birds in Finland.

Author information

1
Department of Food Hygiene and Environmental Health, Faculty of Veterinary Medicine, University of Helsinki, Helsinki, FI-00014, Finland.
2
Ruralia Institute, Faculty of Agriculture and Forestry, University of Helsinki, Seinäjoki, FI-60320, Finland.
3
EVIRA, Finnish Food Safety Authority, Seinäjoki, FI-60101, Finland.
4
Department of Food Hygiene and Environmental Health, Faculty of Veterinary Medicine, University of Helsinki, Helsinki, FI-00014, Finland rauni.kivisto@helsinki.fi.

Abstract

Poultry is considered a major reservoir and source of human campylobacteriosis but the roles of environmental reservoirs, including wild birds, have not been assessed in depth. In this study, we isolated and characterized Campylobacter jejuni from western jackdaws (n=91, 43%), mallard ducks (n=82, 76%) and pheasants (n=9, 9%). Most of the western jackdaw and mallard duck C. jejuni isolates represented MLST sequence types (STs) that diverged from those previously isolated from human patients and various animal species, whereas all pheasant isolates represented ST-19, a common ST among human patients and other hosts worldwide. Whole-genome MLST revealed that mallard duck ST-2314 and pheasant ST-19 isolates represented bacterial clones that were genetically highly similar to human isolates detected previously. Further analyses revealed that in addition to divergent ClonalFrame genealogy certain genomic characteristics, e.g. novel cdtABC gene cluster and T6SS of the western jackdaw C. jejuni isolates may affect their host-specificity and virulence. Game birds may thus pose a risk for acquiring campylobacteriosis, and therefore, hygienic measures during slaughter and meat handling warrant special attention.IMPORTANCEThe roles of environmental reservoirs, including wild birds, in the molecular epidemiology of Campylobacter jejuni have not been assessed in depth. Our results showed that game birds may pose a risk for acquiring campylobacteriosis, because they had C. jejuni genomotypes highly similar to human isolates detected previously. Therefore, hygienic measures during slaughter and meat handling warrant special attention. On the contrary a unique phylogeny was revealed for the western jackdaw isolates and certain genomic characteristics identified among these isolates are hypothesized to affect their host-specificity and virulence. Comparative genomics within STs, using wgMLST, and phylogenomics are efficient methods to analyze the genomic relationships of C. jejuni isolates.

PMID:
30552190
DOI:
10.1128/AEM.02365-18

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