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Diabetologia. 2019 Feb;62(2):212-222. doi: 10.1007/s00125-018-4772-2. Epub 2018 Dec 14.

Use of human islets to understand islet biology and diabetes: progress, challenges and suggestions.

Hart NJ1,2, Powers AC3,4,5.

Author information

1
Department of Medicine, Division of Diabetes, Endocrinology, and Metabolism, 7465 Medical Research Bldg IV, Vanderbilt University Medical Center, 2215 Garland Avenue, Nashville, TN, 37232-0475, USA.
2
Institute for Cellular Transplantation, College of Medicine, Department of Surgery, Arizona Health Sciences Center, Tucson, AZ, USA.
3
Department of Medicine, Division of Diabetes, Endocrinology, and Metabolism, 7465 Medical Research Bldg IV, Vanderbilt University Medical Center, 2215 Garland Avenue, Nashville, TN, 37232-0475, USA. al.powers@Vanderbilt.edu.
4
Department of Molecular Physiology & Biophysics, Vanderbilt University School of Medicine, Nashville, TN, USA. al.powers@Vanderbilt.edu.
5
VA Tennessee Valley Healthcare, Nashville, TN, USA. al.powers@Vanderbilt.edu.

Abstract

Over the last two decades, improved access to human islets and the development of human islet distribution networks have enabled the use of millions of human islets in hundreds of scientific research projects, leading to a dramatic increase in our understanding of human islet biology. Here we discuss recent scientific advances as well as methodological and experimental challenges that impact human islet quality, experimental outcomes and the reporting of human islets used in scientific publications. In a survey of over 200 scientific publications with human islet experimentation, we found that the reporting of critical information was quite variable, sometimes obscure, and often failed to adequately outline the experiments and results using human islets. As the complexity of human islet research grows, we propose that members of the human islet research ecosystem work together to develop procedures and approaches for accessible and transparent collecting and reporting of crucial human islet characteristics and, through this, enhance collaboration, reproducibility and rigour, leading to further advances in our understanding of human islet biology.

KEYWORDS:

Diabetes; Human; Insulin; Islets; Pancreas; Reporting; Research; Resource; Review; Rigour; Transparency; Unique identifier

PMID:
30547228
PMCID:
PMC6325002
[Available on 2020-02-01]
DOI:
10.1007/s00125-018-4772-2
[Indexed for MEDLINE]

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