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Chem Commun (Camb). 2019 Jan 3;55(4):462-465. doi: 10.1039/c8cc08291j.

Improving NanoCluster Beacon performance by blocking the unlabeled NC probes.

Author information

1
Department of Biomedical Engineering, University of Texas at Austin, Austin, Texas 78712, USA. tim.yeh@austin.utexas.edu.

Abstract

While NanoCluster Beacon (NCB) is a versatile molecular probe, it suffers from a low target-specific signal issue due to impurities. Here we show that adding a "blocker" strand to the reaction can effectively block the nonfunctional probes and enhance the target-specific signal by 14 fold at a 0.1 target/probe ratio.

PMID:
30547174
DOI:
10.1039/c8cc08291j

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