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Vox Sang. 2018 Dec 10. doi: 10.1111/vox.12739. [Epub ahead of print]

Platelet storage properties are associated with donor age: in vitro quality of platelets from young donors and older donors with and without Type 2 diabetes.

Author information

1
Department of Product and Process Development, Sanquin Blood Bank, Amsterdam, the Netherlands.
2
Center for Clinical Transfusion Research, Sanquin, Leiden, the Netherlands.
3
Tytgat Institute, Academic Medical Center, Amsterdam, the Netherlands.
4
Department of Blood Cell Research, Sanquin Research, Amsterdam, the Netherlands.

Abstract

BACKGROUND AND OBJECTIVES:

Previously it has been shown that platelet (PLT) storage performance is consistent by donor. Differences involved metabolic activity, which might be caused by mitochondrial (dys)function, associated with age and age-related diseases like Type 2 diabetes (T2D). We aimed to test PLTs from young donors in comparison with PLTs from older donors with or without diagnosis for T2D.

MATERIALS AND METHODS:

Fifteen whole blood donors <30 year were selected, and single-donor platelet concentrates (sPC) were prepared from buffy coats (BC) and plasma. Also, 2 × 11 sPC were prepared from matched donors >45 years with and without T2D. The sPC were stored for 8 days and analysed at regular intervals for in vitro quality.

RESULTS:

Donors were 24 ± 3, 60 ± 7 (without T2D) and 59 ± 8 (with T2D) years old. All sPC groups had comparable volume and PLT content. On Day 8, sPC from young donors showed higher pH37°C than sPC from older donors (6.84 ± 0.15 vs. 6.40 ± 0.48, P < 0.01), due to lower lactate production. Also, CD62P expression (22.9 ± 7.4 vs. 48.8 ± 24.0%, P < 0.01) and HSR reflected better in vitro quality. PLT storage properties of sPC obtained from T2D donors (pH = 6.51 ± 0.35) were not different from sPC of matched donors (pH = 6.40 ± 0.48). No differences in mitochondrial membrane potential were detected between the groups.

CONCLUSION:

Platelets from young donors exhibited the best storage conditions. On average, PLTs from older donors showed poorer in vitro quality but, considering the sub-optimal storage conditions, the implications for the daily blood bank routine is probably small. No association with T2D was found.

KEYWORDS:

blood components; donor health; donors; platelet concentrates

PMID:
30536565
DOI:
10.1111/vox.12739

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