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Dev Comp Immunol. 2019 Mar;92:238-252. doi: 10.1016/j.dci.2018.12.001. Epub 2018 Dec 4.

Molecular characterisation of immunological memory following homologous or heterologous challenges in the schistosomiasis vector snail, Biomphalaria glabrata.

Author information

1
Univ. Perpignan Via Domitia, Interactions Hôtes Pathogènes Environments UMR 5244, CNRS, IFREMER, Univ. Montpellier, F-66860, Perpignan, France. Electronic address: silvainpinaud@me.com.
2
Univ. Perpignan Via Domitia, Interactions Hôtes Pathogènes Environments UMR 5244, CNRS, IFREMER, Univ. Montpellier, F-66860, Perpignan, France. Electronic address: anaisportet@aol.fr.
3
Univ. Perpignan Via Domitia, Interactions Hôtes Pathogènes Environments UMR 5244, CNRS, IFREMER, Univ. Montpellier, F-66860, Perpignan, France. Electronic address: allienne@univ-perp.fr.
4
CEA-Grenoble, Exploring the Dynamics of Proteomes (EDyP), F-38054, Grenoble, Cedex 9, France. Electronic address: Lucid.BELMUDES@cea.fr.
5
Univ. Perpignan Via Domitia, Interactions Hôtes Pathogènes Environments UMR 5244, CNRS, IFREMER, Univ. Montpellier, F-66860, Perpignan, France. Electronic address: cecile.saintbeat@gmail.com.
6
Univ. Perpignan Via Domitia, Interactions Hôtes Pathogènes Environments UMR 5244, CNRS, IFREMER, Univ. Montpellier, F-66860, Perpignan, France. Electronic address: nathalie.arancibia@univ-perp.fr.
7
Univ. Perpignan Via Domitia, Interactions Hôtes Pathogènes Environments UMR 5244, CNRS, IFREMER, Univ. Montpellier, F-66860, Perpignan, France. Electronic address: richard.galinier@univ-perp.fr.
8
University of Basel, Zoological Institute, Department of Zoology and Evolutionary Biology Vesalgasse 1, Basel, Switzerland. Electronic address: louis.dupasquier@unibas.ch.
9
Univ. Perpignan Via Domitia, Interactions Hôtes Pathogènes Environments UMR 5244, CNRS, IFREMER, Univ. Montpellier, F-66860, Perpignan, France. Electronic address: david.duval@univ-perp.fr.
10
Univ. Perpignan Via Domitia, Interactions Hôtes Pathogènes Environments UMR 5244, CNRS, IFREMER, Univ. Montpellier, F-66860, Perpignan, France. Electronic address: benjamin.gourbal@univ-perp.fr.

Abstract

Invertebrate immune response may be primed by a current infection in a sustained manner, leading to the failure of a secondary infection with the same pathogen. The present study focuses on the Schistosomiasis vector snail Biomphalaria glabrata, in which a specific genotype-dependent immunological memory was demonstrated as a shift from a cellular to a humoral immune response. Herein, we investigate the complex molecular bases associated with this genotype-dependant immunological memory response. We demonstrate that Biomphalaria regulates a polymorphic set of immune recognition molecules and immune effector repertoires to respond to different strains of Schistosoma parasites. These results suggest a combinatorial usage of pathogen recognition receptors (PRRs) that distinguish different strains of parasites during the acquisition of immunological memory. Immunizations also show that snails become resistant after exposure to parasite extracts. Hemolymph transfer and a label-free proteomic analysis proved that circulating hemolymph compounds can be produced and released to more efficiently kill the newly encountered parasite of the same genetic lineage.

KEYWORDS:

Biomphalaria; Immunological memory; Proteomic; RNAseq; Schistosoma; Specificity; Vaccination

PMID:
30529491
DOI:
10.1016/j.dci.2018.12.001
[Indexed for MEDLINE]

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