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Dev Cell. 2019 Jan 7;48(1):87-99.e6. doi: 10.1016/j.devcel.2018.11.014. Epub 2018 Dec 6.

Wounding-Induced Stomatal Closure Requires Jasmonate-Mediated Activation of GORK K+ Channels by a Ca2+ Sensor-Kinase CBL1-CIPK5 Complex.

Author information

1
Molekulare Pflanzenphysiologie & Biophysik, Julius-Maximilians-Universität Würzburg, Julius-von-Sachs-Platz 2, Würzburg 97082, Germany.
2
Institut für Biologie und Biotechnologie der Pflanzen, Westfälische Wilhelms-Universität Münster, Münster 48149, Germany.
3
Centre for Organismal Studies, Ruprecht-Karls-Universität Heidelberg, Heidelberg 69120, Germany.
4
CNRS, Laboratoire Reproduction et Développement des Plantes, University Lyon, ENS de Lyon, UCB Lyon 1, INRA, Lyon 69342, France.
5
Instituto de Biologia Molecular y Celular de Plantas, Consejo Superior de Investigaciones Cientificas, Universidad Politecnica de Valencia, Valencia 46022, Spain.
6
Lehrstuhl für Botanik, Technische Universität München, Emil-Ramann Straße 4, Freising 85354, Germany.
7
Molekulare Pflanzenphysiologie & Biophysik, Julius-Maximilians-Universität Würzburg, Julius-von-Sachs-Platz 2, Würzburg 97082, Germany. Electronic address: dirk.becker@uni-wuerzburg.de.

Abstract

Guard cells integrate various hormone signals and environmental cues to balance plant gas exchange and transpiration. The wounding-associated hormone jasmonic acid (JA) and the drought hormone abscisic acid (ABA) both trigger stomatal closure. In contrast to ABA however, the molecular mechanisms of JA-induced stomatal closure have remained largely elusive. Here, we identify a fast signaling pathway for JA targeting the K+ efflux channel GORK. Wounding triggers both local and systemic stomatal closure by activation of the JA signaling cascade followed by GORK phosphorylation and activation through CBL1-CIPK5 Ca2+ sensor-kinase complexes. GORK activation strictly depends on plasma membrane targeting and Ca2+ binding of CBL1-CIPK5 complexes. Accordingly, in gork, cbl1, and cipk5 mutants, JA-induced stomatal closure is specifically abolished. The ABA-coreceptor ABI2 counteracts CBL1-CIPK5-dependent GORK activation. Hence, JA-induced Ca2+ signaling in response to biotic stress converges with the ABA-mediated drought stress pathway to facilitate GORK-mediated stomatal closure upon wounding.

KEYWORDS:

abscisic acid; arabidopsis; calcium; guard cell; jasmonic acid; kinase; patch-clamp; phosphorylation; potassium channel; wounding

PMID:
30528785
DOI:
10.1016/j.devcel.2018.11.014
[Indexed for MEDLINE]
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