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Epilepsia Open. 2018 Nov 2;3(4):460-473. doi: 10.1002/epi4.12277. eCollection 2018 Dec.

How do we use in vitro models to understand epileptiform and ictal activity? A report of the TASK1-WG4 group of the ILAE/AES Joint Translational Task Force.

Author information

1
Department of Neuroscience Tufts University School of Medicine Boston Massachusetts U.S.A.
2
Flocel Inc. and Case Western Reserve University Cleveland Ohio U.S.A.
3
Department of Developmental Epileptology Institute of Physiology of the Czech Academy of Sciences Prague Czechia.
4
Division of Cell Biology and Neuroscience Institute Department of Human Biology Faculty of Health Sciences University of Cape Town Cape Town South Africa.
5
Department of Epilepsy, Movement Disorders and Physiology Kyoto University Graduate School of Medicine Kyoto Japan.
6
Department of Neurology National Cheng Kung University Hospital College of Medicine National Cheng Kung University Tainan Taiwan.
7
The Departments of Neurology and Pediatrics University of Virginia Charlottesville Virginia U.S.A.
8
Laboratory of Developmental Epilepsy Saul R. Korey Department of Neurology Isabelle Rapin Division of Child Neurology Dominick P. Purpura Department of Neuroscience Albert Einstein College of Medicine, and Einstein/Montefiore Epilepsy Center Montefiore Medical Center Bronx New York U.S.A.
9
Aix Marseille Univ, INSERM, INS, Inst Neurosci Syst Marseille France.
10
Epilepsy Unit Fondazione IRCCS Istituto Neurologico Carlo Besta Milano Italy.

Abstract

In vitro brain tissue preparations allow the convenient and affordable study of brain networks and have allowed us to garner molecular, cellular, and electrophysiologic insights into brain function with a detail not achievable in vivo. Preparations from both rodent and human postsurgical tissue have been utilized to generate in vitro electrical activity similar to electrographic activity seen in patients with epilepsy. A great deal of knowledge about how brain networks generate various forms of epileptiform activity has been gained, but due to the multiple in vitro models and manipulations used, there is a need for a standardization across studies. Here, we describe epileptiform patterns generated using in vitro brain preparations, focusing on issues and best practices pertaining to recording, reporting, and interpretation of the electrophysiologic patterns observed. We also discuss criteria for defining in vitro seizure-like patterns (i.e., ictal) and interictal discharges. Unifying terminologies and definitions are proposed. We suggest a set of best practices for reporting in vitro studies to favor both efficient across-lab comparisons and translation to in vivo models and human studies.

KEYWORDS:

Ictal activity; In vitro models; Review

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