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J Med Primatol. 2019 Apr;48(2):99-105. doi: 10.1111/jmp.12395. Epub 2018 Dec 6.

Molecular detection of Bifidobacterium spp. in faeces of black howler monkeys (Alouatta pigra).

Author information

1
Doctorado en Ciencias Agropecuarias, Universidad Autónoma Metropolitana-Xochimilco, Coyoacán, CDMX, México.
2
Instituto de Ecología, A.C. Red de Biología y Conservación de Vertebrados, Xalapa, Veracruz, México.
3
Instituto de Ecología, A.C. Red de Biología Evolutiva, Xalapa, Veracruz, México.
4
Centro de Ciencias Genómicas, Universidad Nacional Autónoma de México, Cuernavaca, Morelos, México.
5
Sistemas Biológicos, Laboratorio de Biotecnología, Universidad Autónoma Metropolitana-Xochimilco, Coyoacán, CDMX, México.

Abstract

BACKGROUND:

Bifidobacterium genus are considered to be beneficial bacteria for their hosts; however, knowledge about the specific species that are part of the gut microbiome of howler monkeys is scarce. Polymerase chain reaction (PCR) is a useful technique for the identification of non-cultivable or difficult to grow bacterial species. With the goal of detecting species of the genus Bifidobacterium in black howler monkeys, we used PCR on DNA derived from faecal samples.

METHODS:

We collected and extracted DNA from 40 faecal samples. Using specific primers, we performed PCR and nested PCR to detect members of the Bifidobacterium genus and a subset of species: Bifidobacterium adolescentis, Bifidobacterium bifidum, Bifidobacterium infantis, Bifidobacterium longum and Bifidobacterium animalis subsp. animalis.

RESULTS:

97.5% (39/40) of the samples were positive for Bifidobacterium spp. We found B longum in 100% of the analysed samples.

CONCLUSIONS:

This is the first report of B longum in black howler monkey faeces.

KEYWORDS:

Alouatta pigra ; Bifidobacterium longum ; PCR; Tabasco; probiotics

PMID:
30520095
DOI:
10.1111/jmp.12395

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