Format

Send to

Choose Destination
Biotech Histochem. 2018 Dec 5:1-9. doi: 10.1080/10520295.2018.1544376. [Epub ahead of print]

Rat lung decellularization using chemical detergents for lung tissue engineering.

Author information

1
a Department of Tissue Engineering and Regenerative Medicine, Nanobiotechnology Research Center , Baqiyatallah University of Medical Sciences , Tehran , Iran.
2
b Research Center for Prevention of Oral and Dental Diseases , Baqiyatallah University of Medical Sciences , Tehran , Iran.
3
c Department of General Surgery, Faculty of Medicine , Baqiyatallah University of Medical Sciences , Tehran , Iran.
4
d Cellular and Molecular Research Center , Iran University of Medical Sciences , Tehran , Iran.
5
e Department of Tissue Engineering & Regenerative Medicine, Faculty of Advanced Technologies in Medicine , Iran University of Medical Sciences , Tehran , Iran.
6
f Cell Therapy and Regenerative Medicine Research Center, Endocrinology and Metabolism Cellular-Molecular Sciences Institute , Tehran University of Medical Sciences , Tehran , Iran.

Abstract

Although pulmonary diseases account for a large number of deaths in the world, most have no treatment other than transplantation. New therapeutic methods for lung treatment include lung tissue engineering and regenerative medicine. Lung decellularization has been used to produce an appropriate scaffold for recellularization and implantation. We investigated 3-[(3-cholamidopropyl) dimethylammonio]-1-propanesulfonate (CHAPS) with sodium dodecyl sulfate (SDS) and Triton X-100 detergents for effecting rat lung decellularization. We evaluated using conventional histology, immunofluorescence staining and SEM methods for removing nuclear material while leaving intact extracellular matrix proteins and three-dimensional architecture. We investigated different concentrations of CHAPS, SDS and Triton X-100 for different periods. We found that 2 mM CHAPS + 0/1% SDS for 48 h was the best among the treatments investigated. Our method can be used to produce an appropriate scaffold for recellularization by stem cells and for investigations ex vivo and in vivo.

KEYWORDS:

CHAPS; SDS; Triton X-100; decellularization; detergents; lung; rat; tissue engineering

Supplemental Content

Full text links

Icon for Taylor & Francis
Loading ...
Support Center