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Tuberculosis (Edinb). 2018 Dec;113:239-241. doi: 10.1016/j.tube.2018.10.014. Epub 2018 Nov 1.

Characterization of specific CD4 and CD8 T-cell responses in QuantiFERON TB Gold-Plus TB1 and TB2 tubes.

Author information

1
QIAGEN Sciences Inc, 19300 Germantown Rd, Germantown MD 20874, USA. Electronic address: nadia.allen@qiagen.com.
2
ViTi Inc, P.O. Box 52, Portland, OR 97207, USA.
3
QIAGEN Sciences Inc, 19300 Germantown Rd, Germantown MD 20874, USA.

Abstract

The new generation of the IGRA QuantiFERON-TB Gold Plus (QFT-Plus) includes two antigen tubes, TB1 and TB2 which contain specific Mycobacterium tuberculosis peptides designed to stimulate both CD4 and CD8 T-cells. TB1 is designed to target cell mediated responses from CD4 T-cells, while TB2 contains newly designed peptides designed to also stimulate CD8 T-cells. We identified specific CD4 and CD8 T-cell clones that recognize different regions spanning the length of the CFP-10 protein in M. tuberculosisto directly test in QFT-Plus TB1 and TB2 tubes, followed by Interferon-gamma detection by the QFT-Plus ELISA. These clones showed specific responses to the different QFT-Plus tubes, the CD4 T-cell clone showed dose-dependent responses to both TB1 and TB2 tubes, while the CD8 T-cell clones showed specific and targeted responses to the QFT-Plus TB2 tube (>140-fold difference) versus the QFT-Plus TB1 tubes using the QFT-Plus ELISA. This testing provides direct evidence of the specificity of CD8 T-cell mediated response in QFT-Plus TB2 tubes.

KEYWORDS:

CD4 T-cell responses; CD8 T-cell responses; QFT-Plus; QuantiFERON; Tuberculosis (TB)

PMID:
30514508
DOI:
10.1016/j.tube.2018.10.014

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