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Nutrients. 2018 Dec 1;10(12). pii: E1856. doi: 10.3390/nu10121856.

Study of Potential Anti-Inflammatory Effects of Red Wine Extract and Resveratrol through a Modulation of Interleukin-1-Beta in Macrophages.

Author information

1
Université de Bourgogne, F-21000 Dijon, France. paulinechalons@orange.fr.
2
INSERM Research Center U1231⁻Cancer and Adaptative Immune Response Team⁻Bioactive Molecules and Health research group, F-21000 Dijon, France. paulinechalons@orange.fr.
3
Université de Bourgogne, F-21000 Dijon, France. souheila.amor@u-bourgogne.fr.
4
Université de Bourgogne, F-21000 Dijon, France. flaviecourtaut@gmail.com.
5
INSERM Research Center U1231⁻Cancer and Adaptative Immune Response Team⁻Bioactive Molecules and Health research group, F-21000 Dijon, France. flaviecourtaut@gmail.com.
6
Intituto de Investigación y Formación Agraria y Pesquera (IFAPA) Rancho de La Merced, Ctra. Trebujena, 11.471 Jerez de la Frontera (Cadiz), Spain. flaviecourtaut@gmail.com.
7
Université de Bordeaux, Unité de Recherche Œnologie, EA 4577, USC 1366 INRA, Equipe Molécules d'Intérêt Biologique-ISVV, F-33882 Villenave d'Ornon, France. tristan.richard@u-bordeaux.fr.
8
UMR 1260 INSERM Nanomédecine Régénérative, Université de Strasbourg, F-67401 Illkirch, France. cyril.auger@unistra.fr.
9
UMR CNRS 7021-Laboratoire de Bioimagerie et Pathologies-Université de Strasbourg, F-67401 Illkirch-Graffenstaden, France. pchabert@unistra.fr.
10
UMR 1260 INSERM Nanomédecine Régénérative, Université de Strasbourg, F-67401 Illkirch, France. valerie.schini-kerth@unistra.fr.
11
Université de Bourgogne, F-21000 Dijon, France. virginie.aires02@u-bourgogne.fr.
12
INSERM Research Center U1231⁻Cancer and Adaptative Immune Response Team⁻Bioactive Molecules and Health research group, F-21000 Dijon, France. virginie.aires02@u-bourgogne.fr.
13
Université de Bourgogne, F-21000 Dijon, France. dominique.delmas@u-bourgogne.fr.
14
INSERM Research Center U1231⁻Cancer and Adaptative Immune Response Team⁻Bioactive Molecules and Health research group, F-21000 Dijon, France. dominique.delmas@u-bourgogne.fr.

Abstract

Inflammation has been described as an initiator event of major diseases with significant impacts in terms of public health including in cardiovascular disease, autoimmune disorders, eye diseases, age-related diseases, and the occurrence of cancers. A preventive action to reduce the key processes leading to inflammation could be an advantageous approach to reducing these associated pathologies. Many studies have reported the value of polyphenols such as resveratrol in counteracting pro-inflammatory cytokines. We have previously shown the potential of red wine extract (RWE) and the value of its qualitative and quantitative polyphenolic composition to prevent the carcinogenesis process. In this study, we addressed a new effect of RWE in inflammation through a modulation of IL-1β secretion and the NLRP3 inflammasome pathway. NLRP3 inflammasome requires two signals, priming to increase the synthesis of NLRP3 and pro-IL-1β proteins and activation, which activates NLRP3. Inflammasome formation is triggered by a range of substances such as lipopolysaccharide (LPS). Using two different macrophages, one of which does not express the adaptor protein ASC (apoptosis-associated speck-like protein containing a CARD), which is essential to form active inflammasome complexes that produce IL-1β, we show that RWE decreases IL-1 β secretion and gene expression whatever line is used. Moreover, this strong reduction of pro-inflammatory IL-1β is associated with a decrease of NLRP3 and, in J774A, ASC protein expression, which depends on the choice of activator ATP or nigericin.

KEYWORDS:

inflammation; interleukins; polyphenols; red wine extract; resveratrol

PMID:
30513737
PMCID:
PMC6316397
DOI:
10.3390/nu10121856
[Indexed for MEDLINE]
Free PMC Article

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