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J Cell Physiol. 2018 Dec 3. doi: 10.1002/jcp.27883. [Epub ahead of print]

27-Hydroxycholesterol enhanced osteoclastogenesis in lung adenocarcinoma microenvironment.

Author information

1
Department of Hand and Foot Surgery, Shandong Provincial Hospital Affiliated to Shandong University, China.
2
Department of Cardiothoracic Surgery, Gansu Provincial Hospital of TCM, Lanzhou, Jinan, China.
3
Department of Gastrointestinal Surgery, Shandong Provincial Hospital Affiliated to Shandong University, Jinan, China.
4
Department of Thoracic Surgery, Shandong Provincial Hospital Affiliated to Shandong University, Jinan, China.
5
Department of Ultrasound, Shandong Provincial Hospital Affiliated to Shandong University, Jinan, China.
6
Department of Medical Engineering, Shandong Provincial Hospital Affiliated to Shandong University, Jinan, China.
7
Department of Center Laboratory, Shandong Provincial Hospital Affiliated to Shandong University, Jinan, China.

Abstract

27-Hydroxycholesterol (27-HC) has been implicated in the pathological process of estrogen receptor positive breast cancer. However, the role of 27-HC in lung adenocarcinoma is still unclear. Because bone metastasis is a main reason for the high mortality of lung adenocarcinoma, this study aimed to investigate the effect of 27-HC on osteoclastogenesis in lung adenocarcinoma microenvironment. The results showed that the conditioned media (CM) from lung adenocarcinoma cells cocultured with macrophages promoted osteoclast differentiation, which was enhanced by 27-HC. Further investigation showed that CM inhibited miR-139 expression and promoted c-Fos expression. Luciferase reporter assay identified c-Fos as a direct target of miR-139. CM also induced the expression and nuclear translocation of NFATc1 and STAT3 phosphorylation, which was enlarged by 27-HC but was attenuated by miR-139. Coimmunoprecipitation assay demonstrated that 27-HC increased the interaction between NFATc1 and phosphorylated STAT3, which was restricted by miR-139. Chromatin immunoprecipitation assay showed that pSTAT3 could bind to the promoter of c-Fos, c-Fos could bind to the promoter of NFATc1, and both pSTAT3 and NFATc1 could bind to the promoter of Oscar, which were enlarged by 27-HC but were blocked by miR-139. Knockdown of c-Fos mimicked the effect of miR-139. These results suggested that CM, especially containing 27-HC, promoted osteoclastogenesis by inhibiting miR-139 expression and activating the STAT3/c-Fos/NFATc1 pathway.

KEYWORDS:

27-hydroxycholesterol; NFATc1; c-Fos; miR-139; osteoclast

PMID:
30511368
DOI:
10.1002/jcp.27883

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