Format

Send to

Choose Destination
Nat Nanotechnol. 2018 Dec 3. doi: 10.1038/s41565-018-0315-8. [Epub ahead of print]

Nanoscale tweezers for single-cell biopsies.

Author information

1
Department of Chemistry, Imperial College London, London, UK.
2
Department of Electrical and Computer Engineering, University of Minnesota, Minneapolis, MN, USA.
3
Department of Experimental Medicine and Toxicology, Imperial College London, London, UK.
4
Department of Neuroscience, Physiology and Pharmacology, University College London, London, UK.
5
School of Electronic and Electrical Engineering, Pollard Institute, University of Leeds, Leeds, UK.
6
Department of Chemistry, Imperial College London, London, UK. alex.ivanov@imperial.ac.uk.
7
Department of Chemistry, Imperial College London, London, UK. joshua.edel@imperial.ac.uk.

Abstract

Much of the functionality of multicellular systems arises from the spatial organization and dynamic behaviours within and between cells. Current single-cell genomic methods only provide a transcriptional 'snapshot' of individual cells. The real-time analysis and perturbation of living cells would generate a step change in single-cell analysis. Here we describe minimally invasive nanotweezers that can be spatially controlled to extract samples from living cells with single-molecule precision. They consist of two closely spaced electrodes with gaps as small as 10-20 nm, which can be used for the dielectrophoretic trapping of DNA and proteins. Aside from trapping single molecules, we also extract nucleic acids for gene expression analysis from living cells without affecting their viability. Finally, we report on the trapping and extraction of a single mitochondrion. This work bridges the gap between single-molecule/organelle manipulation and cell biology and can ultimately enable a better understanding of living cells.

PMID:
30510280
DOI:
10.1038/s41565-018-0315-8

Supplemental Content

Loading ...
Support Center