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Nat Immunol. 2019 Jan;20(1):18-28. doi: 10.1038/s41590-018-0262-4. Epub 2018 Dec 3.

G3BP1 promotes DNA binding and activation of cGAS.

Author information

1
State Key Laboratory of Toxicology and Medical Countermeasures, Institute of Pharmacology and Toxicology, National Center of Biomedical Analysis, Beijing, China.
2
State Key Laboratory of Proteomics, Institute of Basic Medical Sciences, National Center of Biomedical Analysis, Beijing, China.
3
Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD, USA.
4
State Key Laboratory of Toxicology and Medical Countermeasures, Institute of Pharmacology and Toxicology, National Center of Biomedical Analysis, Beijing, China. zhangxuemin@cashq.ac.cn.
5
State Key Laboratory of Proteomics, Institute of Basic Medical Sciences, National Center of Biomedical Analysis, Beijing, China. zhangxuemin@cashq.ac.cn.
6
Cancer Research Institute of Jilin University, The First Hospital of Jilin University, Changchun, China. zhangxuemin@cashq.ac.cn.
7
State Key Laboratory of Toxicology and Medical Countermeasures, Institute of Pharmacology and Toxicology, National Center of Biomedical Analysis, Beijing, China. tli@ncba.ac.cn.
8
State Key Laboratory of Proteomics, Institute of Basic Medical Sciences, National Center of Biomedical Analysis, Beijing, China. tli@ncba.ac.cn.
9
Cancer Research Institute of Jilin University, The First Hospital of Jilin University, Changchun, China. tli@ncba.ac.cn.

Abstract

Cyclic GMP-AMP synthase (cGAS) is a key sensor responsible for cytosolic DNA detection. Here we report that GTPase-activating protein SH3 domain-binding protein 1 (G3BP1) is critical for DNA sensing and efficient activation of cGAS. G3BP1 enhanced DNA binding of cGAS by promoting the formation of large cGAS complexes. G3BP1 deficiency led to inefficient DNA binding by cGAS and inhibited cGAS-dependent interferon (IFN) production. The G3BP1 inhibitor epigallocatechin gallate (EGCG) disrupted existing G3BP1-cGAS complexes and inhibited DNA-triggered cGAS activation, thereby blocking DNA-induced IFN production both in vivo and in vitro. EGCG administration blunted self DNA-induced autoinflammatory responses in an Aicardi-Goutières syndrome (AGS) mouse model and reduced IFN-stimulated gene expression in cells from a patient with AGS. Thus, our study reveals that G3BP1 physically interacts with and primes cGAS for efficient activation. Furthermore, EGCG-mediated inhibition of G3BP1 provides a potential treatment for cGAS-related autoimmune diseases.

PMID:
30510222
DOI:
10.1038/s41590-018-0262-4
[Indexed for MEDLINE]

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