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Eur J Pharm Sci. 2019 Feb 1;128:158-161. doi: 10.1016/j.ejps.2018.11.036. Epub 2018 Nov 30.

Comparative analysis of the antibacterial and drug-modulatory effect of d-limonene alone and complexed with β-cyclodextrin.

Author information

1
Department of Biological Chemistry, Laboratory of Microbiology and Molecular Biology - LMBM, Regional University of Cariri, Crato, Ceará, Brazil.
2
Department of Biological Sciences, Laboratory of Applied Mycology of Cariri - LMAC, Regional University of Cariri, Crato, Ceará, Brazil.
3
Department of Physiology, Federal University of Sergipe, São Cristóvão, Sergipe, Brazil.
4
Department of Biological Chemistry, Laboratory of Pharmacology and Molecular Chemistry - LFQM, Regional University of Cariri, Crato, Ceará, Brazil.
5
Department of Pharmacy, Federal University of Sergipe, São Cristóvão, Sergipe, Brazil.
6
Department of Biological Chemistry, Laboratory of Simulations and Molecular Spectroscopy - LASEMOL, Regional University of Cariri, Crato, Ceará, Brazil.
7
Department of Biological Chemistry, Laboratory of Microbiology and Molecular Biology - LMBM, Regional University of Cariri, Crato, Ceará, Brazil. Electronic address: hdmcoutinho@urca.br.

Abstract

With the increase in bacterial resistance to antibiotics, many studies have been directed towards finding new agents with antibacterial activity, such as studies with natural products. These products can have antibacterial activity such as d-limonene as described in the literature. The aim of this study was to evaluate the antibacterial activity of d-limonene, isolated and complexed with β-cyclodextrin, and to evaluate its potentiating activity of different antibiotic classes. Antibacterial activity was determined by the broth microdilution method, obtaining in this way the Minimal Inhibitory Concentration (MIC), with the antibiotic modulatory activity being obtained using a sub-inhibitory concentration (MIC/8). d-Limonene showed a MIC equal to 256 μg/mL against standard S. aureus and 512 μg/mL against resistant P. aeruginosa. In the gentamicin modulatory activity, the isolated d-limonene presented synergism against S. aureus and E. coli bacteria. Thus, d-limonene showed relevant clinical antibacterial activity, for both Gram-positive and Gram-negative bacteria as well as a synergistic effect when associated with gentamicin. These results are promising in the combat against bacterial resistance, however further studies are needed to better elucidate the mechanisms of action.

KEYWORDS:

Bacterial resistance; Escherichia coli; Pseudomonas aeruginosa; Staphylococcus aureus

PMID:
30508582
DOI:
10.1016/j.ejps.2018.11.036
[Indexed for MEDLINE]

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